Differential role of hydrogen peroxide in UV-induced signal transduction

  • Min Ding
  • Jingxia Li
  • Stephen S. Leonard
  • Xianglin Shi
  • Max Costa
  • Vincent Castranova
  • Val Vallyathan
  • Chuanshu Huang
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 37)


The present study investigated the differential requirement of ROS in UV-induced activation of these pathways. Exposure of the mouse epidermal C141 cells to UV radiation led to generation of ROS as measured by electron spin resonance (ESR) and by H2O2and 02 •- fluorescence staining assay. Treatment of cells with UV radiation or H2O2also markedly activated Erks, JNKs, p38 kinase and led to increases in phosphorylation of Akt and p70s6k in mouse epidermal JB6 cells. The scavenging of UV-generated H2O2 by N-acety-L-cyteine (NAC, a general antioxidant) or catalase (a specific H2O2 inhibitor) inhibited UV-induced activation of JNKs, p38 kinase, Akt and p70s6k while it did not show any inhibitory effects on Erks activation. Further, pretreatment of cells with sodium formate (an •OH radical scavenger) or superoxide dismutase (02 •- radical scavenger) did not inhibit any of these pathways. These results demonstrate that H2O2 generation is required for UV-induced phosphorylation of Akt and p70s6k and involved in activation of JNKs and p38 kinase, but not Erks. (Mol Cell Biochem 234/235: 81–90, 2002)

Key words

oxidative stress antioxidants oxyradical scavengers MAP kinases 


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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Min Ding
    • 2
  • Jingxia Li
    • 1
  • Stephen S. Leonard
    • 2
  • Xianglin Shi
    • 2
  • Max Costa
    • 1
  • Vincent Castranova
    • 2
  • Val Vallyathan
    • 2
  • Chuanshu Huang
    • 1
    • 3
  1. 1.Nelson Institute of Environmental MedicineNew York University School of MedicineTuxedoUSA
  2. 2.Health Effects Laboratory DivisionNational Institute for Occupational Safety and HealthMorgantownUSA
  3. 3.Nelson Institute of Environmental MedicineNew York University School of MedicineTuxedoUSA

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