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Differential role of hydrogen peroxide in UV-induced signal transduction

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Oxygen/Nitrogen Radicals: Cell Injury and Disease

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 37))

Abstract

The present study investigated the differential requirement of ROS in UV-induced activation of these pathways. Exposure of the mouse epidermal C141 cells to UV radiation led to generation of ROS as measured by electron spin resonance (ESR) and by H2O2and 02 •- fluorescence staining assay. Treatment of cells with UV radiation or H2O2also markedly activated Erks, JNKs, p38 kinase and led to increases in phosphorylation of Akt and p70s6k in mouse epidermal JB6 cells. The scavenging of UV-generated H2O2 by N-acety-L-cyteine (NAC, a general antioxidant) or catalase (a specific H2O2 inhibitor) inhibited UV-induced activation of JNKs, p38 kinase, Akt and p70s6k while it did not show any inhibitory effects on Erks activation. Further, pretreatment of cells with sodium formate (an •OH radical scavenger) or superoxide dismutase (02 •- radical scavenger) did not inhibit any of these pathways. These results demonstrate that H2O2 generation is required for UV-induced phosphorylation of Akt and p70s6k and involved in activation of JNKs and p38 kinase, but not Erks. (Mol Cell Biochem 234/235: 81–90, 2002)

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Author information

Authors and Affiliations

  1. Nelson Institute of Environmental Medicine, New York University School of Medicine, Tuxedo, NY, USA

    Jingxia Li, Max Costa & Chuanshu Huang

  2. Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV, USA

    Min Ding, Stephen S. Leonard, Xianglin Shi, Vincent Castranova & Val Vallyathan

  3. Nelson Institute of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY, 10987, USA

    Chuanshu Huang

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  1. Min Ding
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  1. Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, 1095 Willowdale Road Mall Stop B167, Morgantown, WV, 26505, USA

    Val Vallyathan , Xianglin Shi Ph.D.  & Vince Castranova ,  & 

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Ding, M. et al. (2002). Differential role of hydrogen peroxide in UV-induced signal transduction. In: Vallyathan, V., Shi, X., Castranova, V. (eds) Oxygen/Nitrogen Radicals: Cell Injury and Disease. Developments in Molecular and Cellular Biochemistry, vol 37. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1087-1_9

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  • DOI: https://doi.org/10.1007/978-1-4615-1087-1_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5388-1

  • Online ISBN: 978-1-4615-1087-1

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