Abstract
This paper discussesin vivodetection of nitric oxide (NO) distribution in endotoxin-treated mice using L-band (1.1 GHz) electron paramagnetic resonance spectroscopy (EPR) in combination with the hydrophilic NO trapping complex: N-methyl-D-glucamine dithiocarbamate and iron (MGD-Fe). MGD-Fe-NO complex is found in the upper abdomen (liver region), lower abdomen (kidney and urinary bladder) and head region of ICR mice. Experiments with nitric oxide synthase (NOS) inhibition and15N-labeled Larginine as NOS substrate verify the origin of trapped NO from L-arginine. However, contribution from a –nonenzymatic’ NO generation pathway can not be ruled out. This paper further examines potential artifacts, which may arise in experiments using dithiocarbamate-iron complexes as NO trapping agents. (Mol Cell Biochem 234/235: 387 392, 2002)
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Komarov, A.M. (2002). In vivo detection of nitric oxide distribution in mice. In: Vallyathan, V., Shi, X., Castranova, V. (eds) Oxygen/Nitrogen Radicals: Cell Injury and Disease. Developments in Molecular and Cellular Biochemistry, vol 37. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1087-1_43
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DOI: https://doi.org/10.1007/978-1-4615-1087-1_43
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