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Oxygen/Nitrogen Radicals: Cell Injury and Disease pp 387–392Cite as

In vivo detection of nitric oxide distribution in mice

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Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 37))

Abstract

This paper discussesin vivodetection of nitric oxide (NO) distribution in endotoxin-treated mice using L-band (1.1 GHz) electron paramagnetic resonance spectroscopy (EPR) in combination with the hydrophilic NO trapping complex: N-methyl-D-glucamine dithiocarbamate and iron (MGD-Fe). MGD-Fe-NO complex is found in the upper abdomen (liver region), lower abdomen (kidney and urinary bladder) and head region of ICR mice. Experiments with nitric oxide synthase (NOS) inhibition and15N-labeled Larginine as NOS substrate verify the origin of trapped NO from L-arginine. However, contribution from a –nonenzymatic’ NO generation pathway can not be ruled out. This paper further examines potential artifacts, which may arise in experiments using dithiocarbamate-iron complexes as NO trapping agents. (Mol Cell Biochem 234/235: 387 392, 2002)

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Authors and Affiliations

  1. Department of Physiology and Experimental Medicine, The George Washington University Medical Center, Washington, DC, USA

    Andrei M. Komarov

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  1. Andrei M. Komarov
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  1. Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, 1095 Willowdale Road Mall Stop B167, Morgantown, WV, 26505, USA

    Val Vallyathan , Xianglin Shi Ph.D.  & Vince Castranova ,  & 

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Komarov, A.M. (2002). In vivo detection of nitric oxide distribution in mice. In: Vallyathan, V., Shi, X., Castranova, V. (eds) Oxygen/Nitrogen Radicals: Cell Injury and Disease. Developments in Molecular and Cellular Biochemistry, vol 37. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1087-1_43

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  • DOI: https://doi.org/10.1007/978-1-4615-1087-1_43

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5388-1

  • Online ISBN: 978-1-4615-1087-1

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