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In vivo detection of nitric oxide distribution in mice

  • Andrei M. Komarov
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Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 37)

Abstract

This paper discussesin vivodetection of nitric oxide (NO) distribution in endotoxin-treated mice using L-band (1.1 GHz) electron paramagnetic resonance spectroscopy (EPR) in combination with the hydrophilic NO trapping complex: N-methyl-D-glucamine dithiocarbamate and iron (MGD-Fe). MGD-Fe-NO complex is found in the upper abdomen (liver region), lower abdomen (kidney and urinary bladder) and head region of ICR mice. Experiments with nitric oxide synthase (NOS) inhibition and15N-labeled Larginine as NOS substrate verify the origin of trapped NO from L-arginine. However, contribution from a –nonenzymatic’ NO generation pathway can not be ruled out. This paper further examines potential artifacts, which may arise in experiments using dithiocarbamate-iron complexes as NO trapping agents. (Mol Cell Biochem 234/235: 387 392, 2002)

Key words

diethyldithiocarbamate endotoxic shock inducible nitric oxide synthase in vivo EPR nitric oxide spin trapping 

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Andrei M. Komarov
    • 1
  1. 1.Department of Physiology and Experimental MedicineThe George Washington University Medical CenterWashingtonUSA

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