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Measurements in vivo of parameters pertinent to ROS/RNS using EPR spectroscopy

  • Chapter
Oxygen/Nitrogen Radicals: Cell Injury and Disease

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 37))

Abstract

The technique ofin vivoEPR spectroscopy can provide useful and even unique information pertinent to the study of oxygen/ nitrogen radicals and related processes. The parameters that can be measured include: (a) Oxygen centered radicals (by spin trapping); (b) carbon centered radicals (by spin trapping and sometimes by direct observation); (c) sulfur centered radicals (by spin trapping and sometimes by direct observation); (d) nitric oxide (by spin trapping); (e) oxygen (using oxygen sensitive paramagnetic materials); (f) redox state (using metabolism of nitroxides); (g) thiol groups (using special nitroxides); (h) pH (using special nitroxides); (h) perfusion (using washout of paramagnetic tracers); (i) some redox active metal ions (chromium, manganese). The current state of the art for these and other measurements is discussed, especially in relationship to experiments that are likely to be useful for studies of reactive oxygen species (ROS) and/or reactive nitrogen species (RNS). (Mol Cell Biochem 234/235:341-357, 2002)

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Authors and Affiliations

  1. EPR Center, Department of Diagnostic Radiology, Dartmouth Medical School, Hanover, NH, USA

    Nadeem Khan & Harold Swartz

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  1. Nadeem Khan
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  2. Harold Swartz
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  1. Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, 1095 Willowdale Road Mall Stop B167, Morgantown, WV, 26505, USA

    Val Vallyathan , Xianglin Shi Ph.D.  & Vince Castranova ,  & 

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Khan, N., Swartz, H. (2002). Measurements in vivo of parameters pertinent to ROS/RNS using EPR spectroscopy. In: Vallyathan, V., Shi, X., Castranova, V. (eds) Oxygen/Nitrogen Radicals: Cell Injury and Disease. Developments in Molecular and Cellular Biochemistry, vol 37. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1087-1_39

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  • DOI: https://doi.org/10.1007/978-1-4615-1087-1_39

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