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Role of nitric oxide in liver ischemia and reperfusion injury

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Oxygen/Nitrogen Radicals: Cell Injury and Disease

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 37))

Abstract

The present study was designed to assess the role of endothelial cell and inducible nitric oxide synthase (eNOS, iNOS)-derived NO in ischemia/reperfusion (I/R)-induced pro-inflammatory cytokine expression and tissue injury in a murine model of hepatic I/R. Forty-five min of partial hepatic ischemia and 3 h of reperfusion resulted in a significant increase in liver injury as assessed by serum alanine aminotransferase and histopathology which occurred in the absence of neutrophil infiltration. Both iNOS and eNOS deficient mice exhibited enhanced liver injury when compared to their wild type (wt) controls again in the absence of neutrophil infiltration. Interestingly, message expression for both tumor necrosis factor-alpha (TNF-a) and interleukin 12 (IL-12) were enhanced in eNOS, but not iNOS-deficient mice at 1 h post-ischemia when compared to their wt controls. In addition, eNOS message expression appeared to be up-regulated between 1 and 3 h of reperfusion in wt mice while iNOS deficient mice exhibited substantial increases at 1 but not 3 h. Taken together, these data demonstrate the ability of eNOS and iNOS to protect the post-ischemic liver, however their mechanisms of action may be very different. (Mol Cell Biochem 234/235: 229–237,2002)

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Author information

Authors and Affiliations

  1. Departments of Molecular and Cellular Physiology, Shreveport, LA, USA

    Ian N. Hines, Shigeyuki Kawachi, Shigeyuki Harada, Kevin P. Pavlick, Jason M. Hoffman & Matthew B. Grisham

  2. Departments of Pediatrics; Medicine, USA

    Sulaiman Bharwani

  3. Departments of LSU Health Sciences Center, USA

    Robert E. Wolf

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  1. Ian N. Hines
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  2. Shigeyuki Kawachi
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  3. Shigeyuki Harada
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  4. Kevin P. Pavlick
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  1. Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, 1095 Willowdale Road Mall Stop B167, Morgantown, WV, 26505, USA

    Val Vallyathan , Xianglin Shi Ph.D.  & Vince Castranova ,  & 

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Hines, I.N. et al. (2002). Role of nitric oxide in liver ischemia and reperfusion injury. In: Vallyathan, V., Shi, X., Castranova, V. (eds) Oxygen/Nitrogen Radicals: Cell Injury and Disease. Developments in Molecular and Cellular Biochemistry, vol 37. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1087-1_27

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  • DOI: https://doi.org/10.1007/978-1-4615-1087-1_27

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5388-1

  • Online ISBN: 978-1-4615-1087-1

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