Cyclic oxidation and reduction of protein methionine residues is an important antioxidant mechanism

  • Earl R. Stadtman
  • Jackob Moskovitz
  • Barbara S. Berlett
  • Rodney L. Levine
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 37)


Almost all forms of reactive oxygen species (ROS) oxidize methionine residues of proteins to a mixture of the R- and S-isomers of methionine sulfoxide. Because organisms contain methionine sulfoxide reductases (Msr’s) that can catalyze the thioredoxin-dependent reduction of the sulfoxides back to methionine, it was proposed that the cyclic oxidation/reduction of methionine residues might serve as antioxidants to scavenge ROS, and also to facilitate the regulation of critical enzyme activities. We summarize here results of studies showing that organisms possess two different forms of Msr — namely, MsrA that catalyzes reduction of the S-isomer and MsrB that catalyzes the reduction of the R-isomer. Deletion of themsrAgene in mice leads to increased sensitivity to oxidative stress and to a decrease (40%) in the maximum lifespan. This suggests that elimination of both Msr’s would have more serious consequences. (Mol Cell Biochem 234/235: 3–9, 2002)

Key words

methionine sulfoxide reductase antioxidants oxidative stress 


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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Earl R. Stadtman
    • 1
  • Jackob Moskovitz
    • 1
  • Barbara S. Berlett
    • 1
  • Rodney L. Levine
    • 1
  1. 1.Laboratory of BiochemistryNational Heart, Lung, and Blood Institute, National Institutes of HealthUSA

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