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Developmental Changes of Sarcoplasmic Reticular Calcium Ion Transport and Phospholamban in Rat Heart

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Cardiac Development

Part of the book series: Progress in Experimental Cardiology ((PREC,volume 4))

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Summary

This comparative study investigates the relationship between sarcoplasmic reticulum (SR) calcium (Ca2+) ATPase transport activity and phospholamban (PLB) phosphorylation in whole cardiac homogenates around birth and during postnatal development until weaning. At fetal day 20, the rate of homogenate oxalate-supported Ca2+ uptake was 23% of that at postnatal day 6 (0.34 ± 0.08 vs 1.46 ± 0.37 nmoles Ca2+/mg wet ventricular weight/min, respectively; P < 0.05). This was accompanied by a 2-fold gain in heart mass. Between postnatal days 6 and 21, there occurred a further 4-fold increase in heart mass which was accompanied by only a 1.3-fold increase in SR Ca2+ transport activity. Levels of phosphorylated PLB formed in vitro in the presence of radiolabelled ATP and catalytic subunit of protein kinase A increased approx. 4-fold between fetal day 20 and postnatal day 21. In this early developmental period, SR Ca2+-transport values were linearly related to the respective 32P-PLB levels suggesting coordinated developmental changes of SR Ca2+ ATPase and PLB. Developmental changes in SR Ca2+ transport and 32P-PLB levels were thyroid hormone(TH)-dependent as revealed from analysis of these parameters in 21-day-old rats with experimentally induced eu-, hypo- and hyperthyroid states. It appears that TH-dependent changes in the phosphorylation of PLB play a major role for the increase in SR Ca2+ transport activity between birth and weaning.

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Author information

Authors and Affiliations

  1. Institute of Clinical Pharmacology and Toxicology, Benjamin Franklin Medical Center, Freie Universität Berlin, Germany

    Roland Vetter MD, Uwe Rehfeld, Christoph Reissfelder, Wolfgang Weiß & Martin Paul

  2. Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    František Kolář

  3. Institute of Clinical Pharmacology and Toxicology, Benjamin Franklin Medical Center, Freie Universität Berlin, Garystrasse 5, D-14195, Berlin (Dahlem), Germany

    Roland Vetter MD

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  1. Roland Vetter MD
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Editor information

Editors and Affiliations

  1. Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic

    Bohuslav Ostadal M.D., D.Sc. (Professor) (Professor)

  2. Jikei University School of Medicine, Tokyo, Japan

    Makoto Nagano M.D., Ph.D. (Professor Emeritus) (Professor Emeritus)

  3. Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla Ph.D., M.D. (Hon.), D.Sc. (Hon.) (Distinguished Professor and Director) (Distinguished Professor and Director)

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© 2002 Springer Science+Business Media New York

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Vetter, R., Rehfeld, U., Reissfelder, C., Weiß, W., Kolář, F., Paul, M. (2002). Developmental Changes of Sarcoplasmic Reticular Calcium Ion Transport and Phospholamban in Rat Heart. In: Ostadal, B., Nagano, M., Dhalla, N.S. (eds) Cardiac Development. Progress in Experimental Cardiology, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0967-7_11

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  • DOI: https://doi.org/10.1007/978-1-4615-0967-7_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5328-7

  • Online ISBN: 978-1-4615-0967-7

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