Rapid Purification of the T-Protein of the Glycine Cleavage System from Rabbit Liver
The glycine cleavage system (GCS) (E.C. 184.108.40.206) plays a key role in the catabolism of glycine in mammals, birds, reptiles, amphibians and fishes (1). The GCS is a multienzyme complex that catalyzes the reversible cleavage of glycine: Glycine + tetrahydrofolate (THF) + NAD+ ⇔ 5,10-methyleneTHF + CO2 + NH3+NADH (2)
KeywordsGlycerol Sodium Chloride Glycol Glycine Folate
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