Rapid Purification of the T-Protein of the Glycine Cleavage System from Rabbit Liver

  • Montserrat C. Anguera
  • Patrick J. Stover

Abstract

The glycine cleavage system (GCS) (E.C. 2.1.2.10) plays a key role in the catabolism of glycine in mammals, birds, reptiles, amphibians and fishes (1). The GCS is a multienzyme complex that catalyzes the reversible cleavage of glycine: Glycine + tetrahydrofolate (THF) + NAD+ ⇔ 5,10-methyleneTHF + CO2 + NH3+NADH (2)

Keywords

Glycerol Sodium Chloride Glycol Glycine Folate 

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Montserrat C. Anguera
    • 1
  • Patrick J. Stover
    • 1
  1. 1.Division of Nutritional SciencesCornell UniversityIthacaUSA

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