Mutation of W457 Alters N-Hydroxy-L-Arginine Oxidation by Inducible no Synthase: A Single Turnover Study
Nitric oxide synthases (NOS) are homodimeric enzymes which catalyze Arg to nitric oxide and citrulline (1). Its subunits contain a N-terminal oxygenase domain which can bind Fe-protoporphyrin IX (heme), Arg, and the essential cofactor 6R-tetrahydrobiopterin (H4B) and a C-terminal reductase domain containing binding sites for FAD, FMN, and NADPH.
KeywordsHPLC Titration Superoxide Arginine Folate
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