Mutation of W457 Alters N-Hydroxy-L-Arginine Oxidation by Inducible no Synthase: A Single Turnover Study

  • Zhi-Qiang Wang
  • Chin-Chuan Wei
  • Jerome Santolini
  • Dennis J. Stuehr

Abstract

Nitric oxide synthases (NOS) are homodimeric enzymes which catalyze Arg to nitric oxide and citrulline (1). Its subunits contain a N-terminal oxygenase domain which can bind Fe-protoporphyrin IX (heme), Arg, and the essential cofactor 6R-tetrahydrobiopterin (H4B) and a C-terminal reductase domain containing binding sites for FAD, FMN, and NADPH.

Keywords

HPLC Titration Superoxide Arginine Folate 

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Zhi-Qiang Wang
    • 1
  • Chin-Chuan Wei
    • 1
  • Jerome Santolini
    • 1
  • Dennis J. Stuehr
    • 1
  1. 1.Department of ImmunologyLerner Research Institute, Cleveland Clinic FoundationClevelandUSA

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