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Evidence for a Cryptic Gene that Enables E. Coli to Specifically Transport Folate Analogs

  • Jacalyn M. Green
  • Brian P. Nichols

Abstract

The effectiveness of the sulfonamide antibiotics has been attributed to the inability of most bacteria to utilize environmental folate. Escherichia coli and other bacteria depend on de novo synthesis of folate. We have previously isolated pabA- mutants that can grow on p-aminobenzoyl glutamate, a breakdown product of folic acid (Hussein, Green, and Nichols, J. Bact. 1998, 180, 6260–6268). Growth correlated with overproduction of AbgT, an apparent transporter located at the end of a sequence resembling an operon. Using measurements of minimal inhibitory concentration (MIC) for a variety of drugs, we show that over-expression of abgT in wild-type cells imparts a large increase in sensitivities to aminopterin and methotrexate. Inhibition seems to be specific for folate analogs, as there was little difference in the MIC values for other drugs, including trimethoprim, tetracycline, nalidixic acid, kanamycin, rifampicin, chloramphenicol, salicylic acid, and streptomycin. The abg locus may be cryptic for transport of folate analogs or end products of folate catabolism.

Keywords

Salicylic Acid Minimal Inhibitory Concentration Nalidixic Acid Lower Minimal Inhibitory Concentration Sulfonamide Antibiotic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Jacalyn M. Green
    • 1
  • Brian P. Nichols
    • 2
  1. 1.Department of BiochemistryMidwestern UniversityDowners GroveUSA
  2. 2.Laboratory for Molecular Biology, Department of Biological SciencesUniversity of Illinois at ChicagoChicagoUSA

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