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Cr (Vi) Increases Tyrosine Phosphorylation Through Reactive Oxygen Species-Mediated Reactions

  • Yong Qian
  • Bing-Hua Jiang
  • Daniel C. Flynn
  • Stephen S. Leonard
  • Suiwei Wang
  • Zhuo Zhang
  • Jiangping Ye
  • Fei Chen
  • Liying Wang
  • Xianglin Shi
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 34)

Abstract

While Cr (VI)-containing compounds are well established carcinogens, the mechanisms of their action remain to be investigated. In this study we show that Cr (VI) causes increased tyrosine phosphorylation in human lung epithelial A549 cells in a time-dependent manner. N-acetyl-cysteine (NAC), a general antioxidant, inhibited Cr (VI)-induced tyrosine phosphorylation. Catalase, a scavenger of H202sodium formate and aspirin, scavengers of hydroxyl radical (+OH), also inhibited the increased tyrosine phosphorylation induced by Cr (VI). SOD, an inhibitor of superoxide radical (O2+), caused less inhibition. ESR study shows that incubation of Cr (VI) with the A549 cells generates ‘OH radical. The generation of radical was decreased by addition of catalase and sodium formate, while SOD did not have any inhibitory effect. Oxygen consumption measurements show that addition of Cr (VI) to A549 cells resulted in enhanced molecular oxygen consumption. These results indicate that Cr (VI) can induce an increase in tyrosine phosphorylation. H2O2and ‘OH radicals generated during the process are responsible for the increased tyrosine phosphorylation induced by Cr (VI). (Mol Cell Biochem 222: 199-204, 2001)

Key words:

Cr (VI), tyrosine phosphorylation, A549 cells 

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Yong Qian
    • 1
  • Bing-Hua Jiang
    • 1
  • Daniel C. Flynn
    • 1
  • Stephen S. Leonard
    • 2
  • Suiwei Wang
    • 2
  • Zhuo Zhang
    • 2
  • Jiangping Ye
    • 2
  • Fei Chen
    • 2
  • Liying Wang
    • 2
  • Xianglin Shi
    • 2
  1. 1.Cancer Center and Department of Microbiology and ImmunologySchool of Medicine, West Virginia UniversityMorgantownUSA
  2. 2.Pathology and Physiology Research BranchHealth Effects Laboratory Division, National Institute for Occupational Safety and HealthMorgantownUSA

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