Comparative Evaluation of Different Animal Protein Source in Juveniles of Pleoticus Muelleri (Crustacea, Penaeoidea)

  • Ana Cristina Díaz
  • Jorge L. Fenucci


A study was conducted to evaluate alternative protein supplements that could be used to reduce the cost of formulated diets for shrimp. Marine proteins, such as fish meal, squid meal, and bivalve meal, are considerably more expensive than byproduct meals such as meat and bone meal. The objective of this study was to compare the growth, survival, and body composition of juvenile Pleoticus muelleri fed diets containing different levels of meat and bone meal as a substitute of the marine fish meal. Feeding trials were carried out on early juveniles (0.70 ± 0.05 g initial weight) held in 150 l glass aquaria (33% salinity, 20°C, 12:12 h photoperiod, 20 shrimp/m2 density). Each diet (control, PL1, PL2, PL3, PL4) was tested in three replicate groups of 10 shrimps during 50 days. The juveniles were obtained from hatchery-raised postlarvae (wild broodstock from Mar del Plata, Argentina) at Nagera Station, dependent of the Marine Science Department (Mar del Plata National University, Argentina). Four isoproteic and isolipidic diets (34% crude protein) were prepared to contain 0, 11, 15 and 23% meat and bone meal in substitution of fish meal. The control group was fed with fresh squid mantle. Percentage increment in mean weight varied between 81 and 103%. Survival rates ranged from 50 to 76%. No significant differences were detected in final weight gain or survival among dietary treatments (P < 0.05). Whole body moisture, ash, crude protein, and lipid content of shrimps were not affected by diet (P < 0.05). The feeding experiment suggests that meat and bone meal can be utilized by P. muelleri as a suitable replacement for fish meal in a formulated diet.


Fish Meal Shrimp Farming Bone Meal Freshwater Prawn Meat Meal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. AOAC (1990) Official methods of analysis of the Association of Official Chemists. 15thedition, AOAC Inc., Arlington, Virginia, USAGoogle Scholar
  2. Akiyama DM (1992) Future considerations for shrimp nutrition and the aquaculture feed industry, (pp 198–205) In: Wyban J (ed) Proceedings of the Special Session on Shrimp Farming. World Aquaculture Society, Baton RougeGoogle Scholar
  3. Akiyama DM, Dominy GW and Lawrence AL (1991) Penaeid shrimp nutrition for the commercial feed industry: Revised, (pp 80–98) In: Akiyama DM and Tan RKH (eds) Proceedings of the Aquaculture Feed Processing and Nutrition Workshop, SingaporeGoogle Scholar
  4. Chamberlain GW (1993) Aquaculture trends and feed projections. World Aquaculture 24:19–29Google Scholar
  5. Chamberlain GW (1995) Frontiers in shrimp nutrition research, (pp 108–117) In: Browdy CL and Hopkins JS (eds) Proceedings of the Special Session on Shrimp Farming. World Aquaculture SocietyGoogle Scholar
  6. Cowey CB and Forster JRM (1971) The essential amino acid requirements of the prawn,Palaemon serratus. Mar Biol 10:77–81CrossRefGoogle Scholar
  7. Cruz-Suárez LE, Ricque-Marie D, Martínez-Vega JA and Wesche-Ebeling P (1993) Evaluation of two shrimp by-product meals as protein sources in diets forPenaeus vannamei. Aquaculture 115:53–62CrossRefGoogle Scholar
  8. Fenucci JL and Zein-Eldin ZP (1976) Evaluation of squid mantle meal as a protein source in penaeid nutrition. FAO Technical Conference on Aquaculture, Kyoto, Japan, 76/E.36, 9 pGoogle Scholar
  9. Jory DE (1995) Feed management practices for a healthy pond environment, (pp 118–143) In:Browdy CL and Hopkins JS (eds) Proceeding of the Special Session on Shrimp Farming World Aquaculture SocietyGoogle Scholar
  10. Kanazawa A and Teshima S (1981) Essential amino acids of the prawn. Nippon Suisan Gakkaishi 47:1375–1377CrossRefGoogle Scholar
  11. Lim C (1996) Substitution of cottonseed meal for marine animal protein in diets forPenaeus vannamei. J World Aquaculture Soc 27:402–409CrossRefGoogle Scholar
  12. Lovell T (1989) Nutrition and feeding of fish. Ed. Van Nostrand Reinhold, New YorkCrossRefGoogle Scholar
  13. Piedad-Pascual F, Cruz EM and Sumalangcay Jr A (1990) Supplemental feeding ofPenaeus monodonjuveniles with diets containing various levels of defatted soybean meal. Aquaculture 89:183–191CrossRefGoogle Scholar
  14. Reigh RC and Ellis SC (1994) Utilization of animalprotein and plant-protein supplements by red swamp crayfishProcambarus clarkifed formulated diets. J World Aquaculture Soc 25:541–552CrossRefGoogle Scholar
  15. Shewbart KL, Meis WL and Ludwig PD (1972) Identification and quantitative analysis of the amino acids present in protein of the brown shrimp, Penaeus aztecus. Mar Biol 16:64–67CrossRefGoogle Scholar
  16. Sokal R and Rohlf J (1995) Biometry. WH Freeman, New YorkGoogle Scholar
  17. Tacon AGJ and Akiyama DM (1997) Feed ingredients. Advances in World Aquaculture 6:411 -472.Google Scholar
  18. Tidwell JH, Webster CD, Clark JA and D’Abramo LR (1993a) Evaluation of distillers dried grains with solubles as an ingredient in diets for pond culture of the freshwater prawnMacrobrachium rosenbergii. J World Aquaculture Soc 24:66–70CrossRefGoogle Scholar
  19. Tidwell JH, Webster CD, Yancey DH and D’Abramo LR (1993b) Partial and total replacement of fish meal with soybean meal and distillers’ by-products in diets for pond culture of the freshwater prawn (Macrobrachium rosenbergii). Aquaculture 118:119–130CrossRefGoogle Scholar
  20. Watanabe T, Pongmaneerat J, Sato S and Takeuchi T (1993) Replacement of fish meal by alternative protein sources in rainbow trout diets. Nippon Suisan Gakkaishi Bulletin 59:1573–1579CrossRefGoogle Scholar
  21. Wijkstrom UN and New MB (1989) Fish for feed: a help or a hindrance to aquaculture in 2000? INFOFISH International 6/89:48–52Google Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Ana Cristina Díaz
    • 1
    • 2
  • Jorge L. Fenucci
    • 1
    • 2
  1. 1.Departamento de Ciencias MarinasUniversidad Nacional de Mar del PlataMar del PlataArgentina
  2. 2.CIC PciaBuenos Aires CONICETArgentina

Personalised recommendations