Abstract
Helper T cell-regulated B cell immunity constitutes a major component of the immune response to many pathogens. Spatially and temporally regulated changes in lymphocyte function and physiology underpin the primary adaptive response to antigen. We have developed flow-cytometric and single-cell RT-PCR based strategies to quantify the antigen-specific Th cell and B cell responses in non-transgenic animals directly ex vivo. Here, we will summarize our studies in the B10.BR murine response to pigeon cytochrome c (PCC) with some emphasis on the cellular complexity of the Th cell-dependent B cell response to the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
C. R. Monks, B. A. Freiberg, H. Kupfer, N. Sciaky and A. Kupfer. Three-dimensional segregation of supramolecular activation clusters in T cells. Nature. (395): 82–6. (1998)
A. Grakoui, S. K. Bromley, C. Sumen, M. M. Davis, A. S. Shaw, P. M. Allen and M. L. Dustin. The immunological synapse: a molecular machine controlling T cell activation. Science. 285: 221–7. (1999)
K. H. Lee, A. D. Holdorf, M. L. Dustin, A. C. Chan, P. M. Allen and A. S. Shaw. T cell receptor signaling precedes immunological synapse formation. Science. (295): 153–942. (2002)
M. G. McHeyzer-Williams, L. J. McHeyzer-Williams, J. Fanelli Panus, G. Bikah, R. R. Pogue-Caley, D. J. Driver and M. D. Eisenbraun. Antigen-specific immunity. Th cell-dependent B cell responses. Immunol Res. (22): 223–36 (2000)
M. G. McHeyzer-Williams and M. M. Davis. Antigen-specific development of primary and memory T cells in vivo. Science. (268): 106–11. (1995)
M. G. McHeyzer-Williams. Visualizing immune responses in vivo. Curr Opin Immunol. (8): 321–6. (1996)
M. G. McHeyzer-Williams, J. D. Altman and M. M. Davis. Tracking antigen-specific helper T cell responses. Curr Opin Immunol. (8): 278–84. (1996)
F. W. Lii, K. Yasutomo, G. B. Goodman, L. J. McHeyzer-Williams, M. G. McHeyzer-Williams, R. N. Germain and J. D. Ashwell. Thymocyte resistance to glucocorticoids leads to antigen-specific unresponsiveness due to “holes” in the T cell repertoire. Immunity. (12): 183–92. (2000)
L. J. McHeyzer-Williams, J. F. Panus, J. A. Mikszta and M. G. McHeyzer-Williams. Evolution of antigen-specific T cell receptors in vivo: preimmune and antigen-driven selection of preferred complementarity-determining region 3 (CDR3) motifs. JExp Med. (189): 1823–38. (1999)
J. D. Altman, P. A. Moss, P. J. Goulder, D. H. Barouch, M. G. McHeyzer-Williams, J. I. Bell, A. J. McMichael and M. M. Davis. Phenotypic analysis of antigen-specific T lymphocytes. Science. (274): 94–6. (1996)
P. A. Savage, J. J. Boniface and M. M. Davis. A kinetic basis for T cell receptor repertoire selection during an immune response. Immunity. (10): 485–92. (1999)
J. F. Panus, L. J. McHeyzer-Williams and M. G. McHeyzer-Williams. Antigen-specific T helper cell function: differential cytokine expression in primary and memory responses. J Exp Med. (192): 130–116. (2000)
K. M. Ansel, L. J. McHeyzer-Williams, V. N. Ngo, M. G. McHeyzer-Williams and J. G. Cyster. In vivo-activated CD4 T cells upregulate CXC chemokine receptor 5 and reprogram their response to lymphoid chemokines. JExp Med. (190): 1123–34. (1999)
F. D. Batista, D. Iber and M. S. Neuberger. B cells acquire antigen from target cells after synapse formation. Nature. (411): 489–94. (2001)
D. C. Hargreaves, P. L. Hyman, T. T. Lu, V. N. Ngo, A. Bidgol, G. Suzuki, Y. R. Zou, D. R. Littman and J. G. Cyster. A coordinated change in chemokine responsiveness guides plasma cell movements. J Exp Med. (194): 45–56. (2001)
P. Garside, E. Ingulli, R. R. Merica, J. G. Johnson, R. J. Noelle and M. K. Jenkins. Visualization of specific B and T lymphocyte interactions in the lymph node. Science. (281): 96–9. (1998)
G. A. Bishop and B. S. Hostager. B lymphocyte activation by contact-mediated interactions with T lymphocytes. Curr Opin Immunol. (13): 278–85. (2001)
A. H. Sharpe and G. J. Freeman. The B7–CD28 superfamily. Nature Rev Immunol. (2): 116–26. (2002)
G. Bikah, R. R. Pogue-Caley, L. J. McHeyzer-Williams and M. G. McHeyzer Williams. Regulating T helper cell immunity through antigen responsiveness and calcium entry. Nat Immunol. (1): 402–12.
B. Zheng, S. Han and G. Kelsoe. T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. JExp Med. (184): 1083–91. (1996)
M. G. McHeyzer-Williams, M. J. McLean, P. A. Lalor and G. J. Nossal. Antigen-driven B cell differentiation in vivo. JExp Med. (178): 295–307. (1993)
M. K. Slifka, R. Antia, J. K. Whitmire and R. Ahmed. Humoral immunity due to long-lived plasma cells. Immunity. (8): 363–72. (1998)
M. G. McHeyzer-Williams and R. Ahmed. B cell memory and the long-lived plasma cell. Curr Opin Immunol. (11): 172–9. (1999)
L. J. McHeyzer-Williams, M. Cool and M. G. McHeyzer-Williams. Antigen-specific B cell memory: expression and replenishment of a novel b220(-) memory b cell compartment. J Exp Med. (191): 114–966.(2000)
L. J. McHeyzer-Williams, D. J. Driver and M. G. McHeyzer-Williams. Germinal center reaction. Curr Opin Hematol. (8): 52–9. (2001)
D. J. Driver, L. J. McHeyzer-Williams, M. Cool, D. B. Stetson and M. G. McHeyzer-Williams. Development and maintenance of a B220- memory B cell compartment. Jlmmunol. (167): 1393–405. (2001)
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer Science+Business Media New York
About this chapter
Cite this chapter
McHeyzer-Williams, L., Malherbe, L., Eisenbraun, M., Driver, D., McHeyzer-Williams, M. (2002). Development of Antigen-Specific Helper T Cell Responses in Vivo. In: Gupta, S., Butcher, E., Paul, W. (eds) Lymphocyte Activation and Immune Regulation IX. Advances in Experimental Medicine and Biology, vol 512. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0757-4_2
Download citation
DOI: https://doi.org/10.1007/978-1-4615-0757-4_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5226-6
Online ISBN: 978-1-4615-0757-4
eBook Packages: Springer Book Archive