Abstract
In the present study, we overexpressed heme oxygenase-1 (HO-1) in rat aortic smooth muscle cells (SMC) by generating a recombinant defective adenovirus containing the rat HO-1 gene (AdHO-1) and examined the effect on SMC proliferation. Infection of SMC with AdHO-1 resulted in a dose-dependent increase in the expression of HO-1 protein. AdHO-1 inhibited growth factor-stimulated SMC proliferation and DNA synthesis. In contrast, the control adenovirus expressing the green fluorescent protein (AdGFP) failed to induce HO-1 expression and had minimal effects on SMC growth. In addition, the exogenous administration of carbon monoxide (CO) mimicked the antiproliferative effect of AdHO-1, whereas biliverdin and iron had no effect. These results demonstrate that HO-1 inhibits vascular SMC proliferation via the production of CO. Adenovirus-mediated transfer of the HO-1 gene may provide an important therapeutic approach in treating fibroproliferative disorders of the vessel wall.
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Liu, XM., Peyton, K.J., Durante, W. (2002). Heme Oxygenase-1 Inhibits Vascular Smooth Muscle Cell Proliferation. In: Abraham, N.G. (eds) Heme Oxygenase in Biology and Medicine. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0741-3_39
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DOI: https://doi.org/10.1007/978-1-4615-0741-3_39
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