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An Introduction to Laser Spectroscopy pp 139–170Cite as

Fluorescence Probes for Biochemical Systems

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Abstract

In the past several years we have witnessed a significant upsurge in the use of fluorescence in biochemical systems. Until recently, fluorescence spectroscopy was mainly a research tool in biochemistry and biophysics. Today, we see fluorescence being used to determine the DNA sequence of genomes, discover new drugs at unprecedented speed by high throughput screening (HTS) and simultaneously watch thousands of genes turn on and off using DNA arrays or gene chips. Because of the sensitivity of fluorescence detection, and the expense and difficulties of handling radioactive substances, there is a continuing trend towards fluorescencebased measurements and tests. As an example, in the medical field, enzymelinked immunoassays (ELISA) and fluorescence polarization immunoassays have become standard diagnostic tests. In cell biology, the movement and localization of intracellular substances are monitored by fluorescence microscopy. Additionally, cell identification and sorting is accomplished by flow cytometry.

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Authors and Affiliations

  1. Center for Fluorescence Spectroscopy, University of Maryland Medical School, 725, W. Lombard St., Baltimore, MD, USA

    Leah Tolosa, Kazimierz Nowaczyk & Joseph Lakowicz

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  1. Leah Tolosa
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  2. Kazimierz Nowaczyk
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  3. Joseph Lakowicz
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Editors and Affiliations

  1. University of East Anglia, Norwich, NR4 7TJ, UK

    David L. Andrews

  2. LaserSharp Corporation, Boston, Massachusetts

    Andrey A. Demidov

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Tolosa, L., Nowaczyk, K., Lakowicz, J. (2002). Fluorescence Probes for Biochemical Systems. In: Andrews, D.L., Demidov, A.A. (eds) An Introduction to Laser Spectroscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0727-7_5

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  • DOI: https://doi.org/10.1007/978-1-4615-0727-7_5

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