Presence of α-and β-Integrin Subunits in Rabbit Lacrimal Gland Acinar Cells Cultured on a Laminin-Rich Matrix

  • J. Peter Gierow
  • Sofia Andersson
  • Elsie C. Sjögren
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 506)


Integrins play critical roles in the interactions between the extracellular matrix and the cell by directing a number of functions, e.g. cell adhesion, cytoskeletal organization, and signal transduction. It is our hypothesis that they are also involved in modulating the regulation of secretion by lacrimal gland acinar cells, either by direct interaction with intracellular signaling cascades, or through modulation of cytoskeletal organization. Relatively little is known about the integrins in the lacrimal gland, but our laboratory is presently surveying for potential candidates capable of modulating secretion by lacrimal acinar cell. We have previously reported the presence of α2 αv, β1 and β4 integrin subunits in cells obtained by a cytospin technique.1, 2 The present study is an expanded survey for integrin subunits using rabbit lacrimal gland acinar cells in primary culture on laminin coated multi-well slides.


Ocular Surface Lacrimal Gland Lymphocytic Infiltration Keratoconjunctivitis Sicca Apparent Inflammation 
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  1. 1.
    J.P. Gierow and E. Sjögren, Integrins in primary cultures of rat and rabbit lacrimal gland acinar cells, Invest. Ophthalmol. Vis. Sci. 39: s339 (1998).Google Scholar
  2. 2.
    J.P. Gierow, E. Sjögren, and G.W. Laurie, Extracellular matrix proteins, integrins and regulated secretion in lacrimal acinar cells, Hygiea 108:1, p108 (1999).Google Scholar
  3. 3.
    J.P. Gierow, R.W. Lambert, and A.K. Mircheff, Fluid phase endocytosis by isolated rabbit lacrimal gland acinar cells, Exp. Eye Res. 60: 511 (1995).CrossRefPubMedGoogle Scholar
  4. 4.
    J.P. Gierow, T. Yang, A. Bekmezian, N. Liu, J.M. Norian, S.A. Kim, S. Rafisolyman, H. Zeng, C.T. Okamoto, R.L. Wood, and A.K. Mircheff, Na-K-ATPase in lacrimal gland acinar cell endosomal system: correcting a case of mistaken identity, Am. J. Physiol. 271 (Cell Physiol. 40): C1685, (1996).Google Scholar
  5. 5.
    S.F. Hamm-Alvarez, S. da Costa, T. Yang, X. Wei, J.P. Gierow, and A.K. Mircheff, Cholinergic Stimulation of lacrimal acinar cells promotes redistribution of membrane-associated kinesin and the secretory protein, hexosaminidase, and activation of soluble kinesin, Exp. Eye Res. 64: 141 (1997).CrossRefPubMedGoogle Scholar
  6. 6.
    M.-N. Saarloos, M.R. Husa, R.S. Jackson, and J.L. Ubels, Intermediate filament, laminin and integrin expression in lacrimal gland acinar cells: Comparison of an immortalized cell line to primary cells, and their response to retinoic acid, Curr. Eye Res. 19: 439 (1999).CrossRefPubMedGoogle Scholar
  7. 7.
    D. Cox, T. Aoki, J. Seki, Y. Motoyama, and K. Yoshida, The pharmacology of the integrins, Med. Res. Rev. 14: 195(1994).CrossRefPubMedGoogle Scholar
  8. 8.
    S.G. Einer and V.M. Einer, The integrin superfamily and the eye, Invest. Ophthalmol. Vis. Sci. 37, 696 (1996).Google Scholar

Copyright information

© Kluwer Academic/Plenum Publishers 2002

Authors and Affiliations

  • J. Peter Gierow
    • 1
  • Sofia Andersson
    • 1
  • Elsie C. Sjögren
    • 1
  1. 1.Department of Chemistry and Biomedical SciencesUniversity of KalmarKalmarSweden

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