Evaluation of the Effects of 15(S)-HETE on Corneal Epithelial Cells: An Electrophysiological and Cytochemical Study

  • Ramzy G. Azar
  • Henry F. Edelhauser
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 506)


The healthy corneal epithelium has tight junctions and forms a barrier between the environment and the inside of the eye. The tear film aids in protecting the corneal surface, and artificial tear solutions provide temporary relief of dry eye. The tear film is composed of three layers: the lipid, aqueous and mucin layers. The mucin layer, which lies adjacent to the ocular surface, provides an evenly distributed hydrophilic coating on the surface of the cornea. The conjunctival goblet cells1 and corneal and conjunctival epithelial cells continually secrete mucin over the corneal surface. When the outer epithelial layer becomes damaged, the epithelial cells below can produce mucin.2,3 A recent theory is that the secretion of mucin may serve as a method of treating dry eye.


Ophthalmic Examination Sodium Fluorescein Oral Antihistamine Chlorpheniramine Maleate Ocular Discomfort 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Greiner J, Weidman T, Korb D, Allansmith M. Histochemical analysis of secretory vesicles in nongoblet conjunctival epithelial cells. Acta Ophthalmol. 1985; 63: 89–92.CrossRefGoogle Scholar
  2. 2.
    Ubels J, McCartney M, Lantz W, Beaird J, Dayalan A, Edelhauser H. Effects of preservative-free artificial tear solutions on the corneal epithelial structure and function. Arch Ophthalmol. 1995; 113: 371–378.CrossRefPubMedGoogle Scholar
  3. 3.
    Gipson I, Yankaukas M, Spurr-Michaud S, Tisdale A, Rinehart W. Characteristics of glycoprotein in the ocular surface glycocalyx. Invest Ophthalmol Vis Sci. 1992;33: 218–277.PubMedGoogle Scholar
  4. 4.
    Johnson H, McNee M, Sun F. 15-hydroxyeicosatetraenoic acid is a potent inflammatory mediator and agonist of canine tracheal mucus secretion. Am Rev Respir Dis. 1985; 131: 917–922.PubMedGoogle Scholar
  5. 5.
    Marom Z, Shelhamer J, Sun F, Kaliner M. Human airway monohydroxy-eicosatetraenoic acid generation and mucus release. J Clin Invest. 1983;72: 122–127.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Copas J, Borgeat P, Gardiner P. The action of 5, 12, and 15 HETE on the tracheobronchial smooth muscle. Prostaglandins Leukot Med. 1982;8: 105–114.CrossRefPubMedGoogle Scholar
  7. 7.
    Klyce S, Neufeld A, Zadunaisky J. The activation of chloride transport by epinephrine and Db cyclic-AMP in the cornea of the rabbit. Invest Ophthalmol. 1973;12: 127–139.PubMedGoogle Scholar
  8. 8.
    Edelhauser H, Rudnick D, Azar R, Corneal epithelial tight junctions and the localization of surface mucin, In Lacrimal Gland, Tear Film and Dry Eye Syndromes 2, D.A. Sullivan, Editor. 1998, Plenum Press: New York. p. 265–271.CrossRefGoogle Scholar
  9. 9.
    Ubels J, Williams K, Lopez-Bernal D, Edelhauser H, Evaluation of effects of a physiological artificial tear on the corneal epithelial barrier: Electrical resistance and carboxyfluorescein permeability, In Lacrimal Gland, Tear Film, and Dry Eye Syndromes, D.A. Sullivan, Editor. 1994, Plenum Press: New York. p. 441–452.CrossRefGoogle Scholar
  10. 10.
    Maurice D. Epithelial potential of the cornea. Exptl Eye Res. 1967;6: 138–140.CrossRefGoogle Scholar
  11. 11.
    Bernai D. Quantitative evaluation of the corneal epithelial barrier: effect of artificial tears and preservatives. CurrEye Res. 1991; 10: 645–656.Google Scholar
  12. 12.
    Burnstein N, Klyce S. Electrophysiologic and morphologic effects of ophthalmic preparations on the rabbit cornea epithelium. Invest Ophthalmol Vis Sci. 1977; 16: 899–911.Google Scholar
  13. 13.
    Nichols B, Chiappino M, Dawson C. Demonstration of the mucous layer of the tear film by electron microscopy. Invest Ophthalmol Vis Sci. 1985;26: 464–473.PubMedGoogle Scholar

Copyright information

© Kluwer Academic/Plenum Publishers 2002

Authors and Affiliations

  • Ramzy G. Azar
    • 1
  • Henry F. Edelhauser
    • 1
  1. 1.Emory Eye CenterEmory UniversityAtlantaUSA

Personalised recommendations