Advertisement

Human Conjunctival Epithelium in Culture: A Tool to Assay New Therapeutic Strategies for Dry Eye

  • Yolanda Diebold
  • Rosa M. Corrales
  • Margarita Calonge
  • M. Victoria Saez
  • Sagrario Callejo
  • M. Carmen Lazaro
  • Jose M. Herreras
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 506)

Abstract

Conjunctival changes in dry eye syndrome involve alteration in the mucous layer of the tear film. Mucus production and secretion is one of the principal roles of the conjunctival epithelium. Mucins, the main components of the mucous layer, are glycoproteins produced by goblet and non-goblet epithelial cells from the conjunctiva1–3 and by corneal epithelial cells.4 Any process that interferes with normal mucin production also interferes with proper wetting of the ocular surface.

Keywords

Hormone Replacement Therapy Lacrimal Gland Lipid Layer Hormone Replacement Therapy Group Tear Film Stability 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    T. Inatomi, S. Spurr-Michaud, A.S. Tisdale, Q. Zhan, S.T. Feldman, and I.K. Gipson. Expression of secretory mucin genes by human conjunctival epithclia. Invest Ophthalmol Vis Sci. 37:684 (1996).Google Scholar
  2. 2.
    R.W. McKenzie, J.E. Jumblatt, and M.M. Jumblatt. Quantification of MUC2 and MUC5AC transcripts in human conjunctiva. Invest Ophthalmol Vis Sci. 41:03 (2000).Google Scholar
  3. 3.
    M. Berry, R.B. Ellingham, A.P. Corfield. Membrane-associated mucins in normal human conjunctiva. Invest Ophthalmol Vis Sci. 41:98 (2000).Google Scholar
  4. 4.
    T. Inatomi, S. Spurr-Michaud, A.S. Tisdale, I.K. Gipson. Human corneal and conjunctival epithelia express MUC1 mucin. Invest Ophthalmol Vis Sci. 36:818 (1995).Google Scholar
  5. 5.
    Y. Diebold, M. Calonge, N. Fernandez, M.C. Lazaro, S. Callejo, J.M. Herreras, and J.C. Pastor. Characterization of epithelial primary cultures from human conjunctiva. Graefe’s Arch Clin Exp Ophthalmol. 235:68 (1997).CrossRefGoogle Scholar
  6. 6.
    Y. Diebold, M. Calonge, S. Callejo, M.C. Lazaro, R. Bringas, and J.M. Herreras. Ultrastructural evidence of mucus in human conjunctival epithelial cultures. Curr Eye Res. 19:5 (1999).CrossRefGoogle Scholar
  7. 7.
    B.A. Nichols, M.L. Chiappino, and CR. Dawson. Demonstration of the mucous layer of the tear film by electron microscopy. Invest Ophthalmol Vis Sci. 26:64 (1985).Google Scholar
  8. 8.
    P. Chomczynski and N. Sacchi. Single step method of RNA isolation by acid guanidinium-thyocianate-phenol-chloroform extraction. Anal Biochem 162:156 (1987).CrossRefPubMedGoogle Scholar
  9. 9.
    I.K. Gipson, S. Spurr-Michaud, R. Moccia, Q. Zhan, N. Toribara, S.B. Ho, et al. MUC4 and MUC5B transcripts are the prevalent mucin messenger ribonucleic acids of the human endocervix. Biol Reprod. 60:58 (1999).CrossRefPubMedGoogle Scholar

Copyright information

© Kluwer Academic/Plenum Publishers 2002

Authors and Affiliations

  • Yolanda Diebold
    • 1
  • Rosa M. Corrales
    • 1
  • Margarita Calonge
    • 1
  • M. Victoria Saez
    • 1
  • Sagrario Callejo
    • 2
  • M. Carmen Lazaro
    • 3
  • Jose M. Herreras
    • 1
  1. 1.Instituto Universitario de Oftalmobiologia Aplicada (IOBA)University of ValladolidValladolidSpain
  2. 2.Human Anatomy DepartmentUniversity of ValladolidValladolidSpain
  3. 3.Instrumental Techniques LaboratoryUniversity of ValladolidValladolidSpain

Personalised recommendations