Advertisement

Metabolism and Transport of Purinergic Receptor Agonists in Rabbit Conjunctival Epithelial Cells

  • Hovhannes J. Gukasyan
  • Benjamin R. Yerxa
  • William Pendergast
  • Vincent H. L. Lee
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 506)

Abstract

The role of extracellular nucleotides as signaling molecules is well established in a number of biological systems. Their function in the regulation of ion1, 2 and fluid3 transport has been investigated in the pigmented rabbit conjunctiva where active Cl secretion has been implicated as the driving force for fluid secretion.3 These agonists interact with two classes of receptors located on the cell membrane: P2X ligand-gated ion channels and P2Y G-protein-coupled receptors.4 Receptor mapping techniques have revealed the presence of P2Y type receptors in ocular epithelial cells.5

Keywords

Contact Lens Protein Profile Protein Deposition Contact Lens Wear Lens Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    U.B. Kompella, K.J. Kim, and V.H. Lee. Active chloride transport in the pigmented rabbit conjunctiva. Curr Eye Res. 12:1041 (1993).CrossRefPubMedGoogle Scholar
  2. 2.
    K. Hosoya, H. Ueda, K.J. Kim, and V.H. Lee. Nucleotide stimulation of Cl- secretion in the pigmented rabbit conjunctiva. J Pharmacol Exp Ther. 291:53 (1999).PubMedGoogle Scholar
  3. 3.
    M.H. Shiue, A.A. Kulkarni, H.J. Gukasyan, J.B. Swisher, K.J. Kim, and V.H. Lee. Pharmacological modulation of fluid secretion in the pigmented rabbit conjunctiva. Life Sci. 66:PL105 (2000).CrossRefGoogle Scholar
  4. 4.
    V. Ralevic and G. Burnstock. Receptors for purines and pyrimidines. Pharmacol Rev. 50:413 (1998).PubMedGoogle Scholar
  5. 5.
    B.R. Yerxa, V.Z. Zhang, L. Sheridan, CF. Moyer, and M.S. Cowlen. Cellular localization of P2Y2 receptor gene expression in primate ocular tissues by nonisotopic in situ hybridization. Invest Ophthalmol Vis Sci. 41:4630-B577 (2000).Google Scholar
  6. 6.
    J.R. Sabater, Y.M. Mao, C. Shaffer, M.K. James, T.G. O’Riordan, and W.M. Abraham. Aerosolization of P2Y2-receptor agonists enhances mucociliary clearance in sheep. J Appl Physiol. 87:2191 (1999).PubMedGoogle Scholar
  7. 7.
    M.V. Mundasad, G.D. Novack, V.E. Allgood, R.M. Evans, J.C. Gorden, and B.R. Yerxa. Ocular safety of INS365 ophthalmic solution: A P2Y2 agonist, in healthy subjects. J Ocul Pharmacol Ther. 17:173 (2001).CrossRefPubMedGoogle Scholar
  8. 8.
    M. Picher and R.C. Boucher. Biochemical evidence for an ecto alkaline phosphodiesterase I in human airways. Am J Respir Cell Mol Biol. 23:255 (2000).CrossRefPubMedGoogle Scholar
  9. 9.
    K. Hosoya, Y. Horibe, K.J. Kim, and V.H. Lee. Nucleoside transport mechanisms in the pigmented rabbit conjunctiva. Invest Ophthalmol Vis Sci. 39:372 (1998).PubMedGoogle Scholar
  10. 10.
    P. Saha, K.J. Kim, and V.H. Lee. A primary culture model of rabbit conjunctival epithelial cells exhibiting tight barrier properties. Curr Eye Res. 15:1163 (1996).CrossRefPubMedGoogle Scholar

Copyright information

© Kluwer Academic/Plenum Publishers 2002

Authors and Affiliations

  • Hovhannes J. Gukasyan
    • 1
  • Benjamin R. Yerxa
    • 2
  • William Pendergast
    • 2
  • Vincent H. L. Lee
    • 1
  1. 1.Departments of Pharmaceutical Sciences and OphthalmologyUniversity of Southern CaliforniaLos AngelesUSA
  2. 2.Inspire PharmaceuticalsDurhamUSA

Personalised recommendations