Advertisement

Immunolocalization and Physiological Effect of Serotonin in the Porcine Lacrimal Gland

  • Ernest Adeghate
  • Benjamin Zumbrunn
  • Jaipaul Singh
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 506)

Abstract

The lacrimal gland produces the bulk of the tear film that covers the external surface of the cornea and ensures that the corneal epithelium is kept moist and transparent. The transparency of the cornea is a prerequisite for normal refraction of light. The lacrimal gland is innervated by a branch of the ophthalmic division of the trigeminal nerve.1 The nerve supply of the lacrimal gland consists of afferent and efferent pathways involving both the sympathetic and parasympathetic divisions of the nervous system. It has been shown that neurotransmitters and neuropeptides are present in the nerves innervating the lacrimal gland of various mammalian species.2, 3 The aim of this study was to examine the effect of serotonin (5-HT) on total protein, peroxidase and magnesium released from pig lacrimal gland fragments. The immunolocalization of 5-HT in the porcine lacrimal glands was investigated using immunohistochemical techniques.

Keywords

High Performance Liquid Chromatography Polar Lipid Contact Lens Cholesterol Ester Lipid Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    S. Y. Bothelo, Tears and the lacrimal gland. Sci Am 211: 78–86 (1964).CrossRefGoogle Scholar
  2. 2.
    E. Adeghate, and J. Singh, Immunohistochemical identification of galanin and leucine-enkephalin in the porcine lacrimal gland. Neuropeptides 27: 285–289 (1994).CrossRefPubMedGoogle Scholar
  3. 3.
    E. Adeghate, Pattern of distribution of neuropeptides in the camel lacrimal gland. Neuropeptides 30: 566–571 (1996).CrossRefPubMedGoogle Scholar
  4. 4.
    O.H. Lowry, N.J. Roseborough, A.L. Farr, and R.J. Randall, Protein measurement with the Folin phenol reagent. J Biol Chem 193: 262–275 (1951).Google Scholar
  5. 5.
    V. Herzog, and H.D. Fahimi, A new and sensitive colorimetric assay of peroxidase using 3’, 3’-diaminobenzidine as a hydrogen donor. Anal Biochem 5: 554–562 (1973).CrossRefGoogle Scholar
  6. 6.
    R. Lennard, and J. Singh, (1991) Secretagogue-evoked changes in intracellular free magnesium concentration in rat pancreatic acinar cells. J Physiol 435:483–492 (1991).CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    E. Adeghate, M. Seker, S. Ponery, I. Ahmed, D.J. Pallot, and H. Parvez, Immunohistochemical localization of vimentin, S-100 protein and neurofilament in the lacrimal gland of the camel (camelus dromedarius). Biogenic Amines 12: 437–444 (1996).Google Scholar
  8. 8.
    M.D. Yago, M. Mañas, and J. Singh, Intracellular free magnesium: regulation and transport in epithelial secretory cells. Frontiers in Bioscience 5: 602–619 (2000).CrossRefGoogle Scholar

Copyright information

© Kluwer Academic/Plenum Publishers 2002

Authors and Affiliations

  • Ernest Adeghate
    • 1
  • Benjamin Zumbrunn
    • 2
  • Jaipaul Singh
    • 2
  1. 1.Department of Human Anatomy, Faculty of Medicine and Health SciencesUnited Arab Emirates UniversityAl AinUnited Arab Emirates
  2. 2.Department of Biological SciencesUniversity of Central LancashirePrestonEngland, UK

Personalised recommendations