Cytoskeletal Participation in Stimulated Secretion and Compensatory Apical Plasma Membrane Retrieval in Lacrimal Gland Acinar Cells
One of the main functions of the lacrimal gland is the regulated secretion of tear fluid, rich in proteins and electrolytes, in response to signals provided through the sympathetic and parasympathetic nervous system. Since proper tear-fluid secretion is essential for maintenance of ocular health, a long-term focus of our laboratory has been to understand the molecular mechanisms governing regulated secretion in lacrimal acini. In particular, we have focused on the role of microtubules (MTs), actin-based microfilaments (MFs), and motor proteins associated with either filament system in the stimulated lacrimal acinar secretory response. MTs and MFs constitute two of the three major cytoskeletal filament systems in mammalian cells, the third system being the intermediate filaments.
KeywordsContact Lens Corneal Epithelium Contact Lens Wear Lens Surface Filter Paper Strip
Unable to display preview. Download preview PDF.
- S.F. Hamm-Alvarez, S.R. da Costa, T. Yang, X. Wei, J. P. Gierow, and A.K Mircheff… Cholinergic stimulation of lacrimal acinar cells promotes redistribution of membrane-associated kinesin and the secretory protein, β-hexosaminidase, and activation of soluble kinesin. Exp Eye Res. 64:141 (1997).CrossRefPubMedGoogle Scholar
- J. Jungerman, M.M. Lerch, H. Weidenbach, M.P. Lutz, B. Kruger and G. Adler. Disassembly of rat pancreatic acinar cell cytoskeleton during supramaximal secretagogue stimulation. Am J Physiol. 31:G328(1995).Google Scholar
- W.J. Nelson.. Cytoskeleton functions in membrane traffic in polarized epithelial cells. Sem Cell Biol. 2:375 (1991).Google Scholar
- P. Robin, B. Rossignol, and M.N. Raymond. Effect of microtubule network disturbance by nocodazole and docetaxel (Taxotere) on protein secretion in rat extraorbital lacrimal and parotid glands. Eur J Cell Biol. 67:221 (1995).Google Scholar