Abstract
We have recently established procedures for the isolation of highly purified lacrimal gland acinar cells (pLGAC) (Guo et al., 2000) and a culture method that promotes proliferation of the cells (Schönthal et al., 2000). Many studies have shown the benefits of seeding lacrimal cells onto Matrigel coated plates (Kelleher et al., 1991; Menerey et al., 1994; Millar, et al., 1996; Chen et al., 1998). Our procedure utilizes a method which coats the pLGAC with Matrigel to more closely mimic the in vivo condition where acinar cells are enveloped by matrix molecules on their basal surfaces. This has been achieved by trapping pLGAC in Matrigel rafts. These free-floating raft cultures have been maintained in vitro for up to 28 days for morphological and physiological studies.
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References
L. Chen, J.D. Glass, S.C. Walton, and G.W. Laurie. Role of laminin-1, collagen IV, and an autocrine factor(s) in regulating secretion by lacrimal acinar cells. Am J Physiol. 275:C278–284 (1998).
S.R. da Costa, F.A. Yarber, L. Zhang, M. Sonee, and S.F. Hamm-Alvarez. Microtubules facilitate the stimulated secretion of beta-hexosaminidase in lacrimal acinar cells. J Cell Sci. 111:1267 (1998).
Z. Guo, A.M. Azzarolo, J.E. Schechter, D.W. Warren, R.L. Wood, A.K. Mircheff, and H.R. Kaslow. Lacrimal gland epithelial cells stimulate proliferation in autologous lymphocyte preparations. Exp Eye Res. 71:11 (1998).
R.S. Kelleher, L.E. Hann, J.A. Edwards, and D.A. Sullivan. Endocrine, neural, and immune control of secretory component output by lacrimal acinar cells. J Immunol. 146:3405 (1991).
M.A. Meneray, T.Y. Fields, B.B. Bromberg, and R.L. Moses. Morphology and physiologic responsiveness of cultured rabbit lacrimal acini. Invest Ophthalmol Vis Sci. 35:4144 (1994).
T.J. Millar, G. Herok, H. Koutavas, D.K. Martin, and P.J. Anderton, P.J. Immunohistochemical and histochemical characterisation of epithelial cells of rabbit lacrimal glands in tissue sections and cell cultures. Tiss Cell 28:301 (1996).
M. Pidgeon, J. Schechter, N. Chang, D. Chang, M. Trousdale and D. Stevenson, The Technical aspects for studying morphology of purified lacrimal gland acinar cells cultured on matrigel rafts: A new model system for studying lacrimal physiology, Microsc. Microanal., 6 (suppl.2), 894–895.
J. Schechter, C. Carey, M. Wallace, and R. Wood. Distribution of growth factors and immune cells are altered in the lacrimal gland during pregnancy and lactation. Exp Eye Res. 71:129 (2000).
A.H. Schönthal, D.W. Warren, D. Stevenson, J.E. Schechter, A.M. Azzarolo, A.K. Mircheff, and M.D. Trousdale. Proliferation of lacrimal gland acinar cells in primary culture. Stimulation by extracellular matrix, EGF and DHT. Exp Eye Res, 70:639 (2000)
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© 2002 Kluwer Academic/Plenum Publishers
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Stevenson, D. et al. (2002). A New Model System for Studying Lacrimal Physiology Using Cultured Lacrimal Gland Acinar Cells on Matrigel® Rafts. In: Sullivan, D.A., Stern, M.E., Tsubota, K., Dartt, D.A., Sullivan, R.M., Bromberg, B.B. (eds) Lacrimal Gland, Tear Film, and Dry Eye Syndromes 3. Advances in Experimental Medicine and Biology, vol 506. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0717-8_21
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DOI: https://doi.org/10.1007/978-1-4615-0717-8_21
Publisher Name: Springer, Boston, MA
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