Abstract
The phytohormone abscisic acid (ABA) regulates diverse developmental and physiological responses, including seed maturation, dormancy and germination as well as guard cell closure. ABA also mediates adaptive responses to abiotic environmental stresses such as drought (Leung and Giraudat, 1998). The role of ABA in cold acclimation has been the center of much debate. However, several lines of evidence suggest that the ABA may have an important role in the cold acclimation process. First, application of ABA at normal growth temperatures can induce an increase in freezing tolerance in a wide range of plants, including Arabidopsis (Guy, 1990 Lång et al., 1989). Furthermore, endogenous ABA levels increase transiently in response to low temperature (Lång et al., 1994). In addition, the ABA-deficient mutants of Arabidopsis, aba1 and aba4, are severely impaired in their ability to cold-acclimate (Heino et al., 1990; Gilmour and Thomashow, 1991). However, application of ABA could suppress the impaired cold-acclimation phenotype (Heino et al., 1990). In addition to mutants in ABA biosynthesis, also Arabidopsis mutants defective in ABA responsiveness appear to affect cold acclimation. The ABA-insensitive mutant abi1 is impaired in development of freezing tolerance (Mäntylä et al., 1995) as well as in the cold-induced expression of several cold-responsive genes (Lång and Palva, 1992; Nordin et al., 1993). Identification of ABI1 as a protein phosphatase 2C (Leung et al., 1994; Meyer et al., 1994) suggested that protein dephosphorylation might be involved in cold signal transduction.
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References
Armstrong, F., Leung, J., Grabov, A., Brearley, J., Giraudat, J. and Blatt, M., 1995, Sensitivity to abscisic acid of guard-cell K+ channels is suppressed by abi1–1, a mutant Arabidopsis gene encoding a putative protein phosphatase, Proc. Natl. Acad. Sci. USA, 92: 9520–9524.
Cao, Y., Ward, J.M., Kelly, W.B., Ichida, A.M., Gaber, R.F., Anderson, J.A., Uozumi, N., Schroeder, J.I. and Crawford, N.M., 1995, Multiple genes, tissue specificity, and expression-dependent modulation contribute to the functional diversity of potassium channels in Arabidopsis thaliana, Plant Physiol. 109: 1093–1106.
Capel, J., Jarillo, J.A., Salinas, J. and Martinez-Zapater, J.M., 1997, Two homologous low-temperature-inducible genes from Arabidopsis encode highly hydrophobic proteins, Plant Physiol. 115: 569–576.
Choi, H-I, Hong, J-H., Ha, J-O., Kang, J-Y. and Kim, S.Y., 2000, ABFs, a femily of ABA-responsive element binding fectors, J. Biol. Chem. 275: 1723–1730.
Cutler, S., Ghassemian, M., Bonetta, D., Cooney S., and McCourt, P., 1996, A protein famesyI transferase involved in abscisic acid signal transduction in Arabidopsis, Science, 273: 1239–1240.
Dennison, K.L., Robertson, W.R., Lewis, B.D., Hirsch, R.E., Sussman, M.R. and Spalding, E.P., 2001, Functions of AKT1 and AKT2 potassium channels determined by studies of single and double mutants of Arabidopsis, Plant Physiol. 127: 1012–1019.
Foster, R., and Chua, N.-H., 1999, An Arabidopsis mutant with deregulated ABA gene expression: implications for negative regulator function, Plant J. 17: 363–372.
Gilmour, S.J. and Thomashow, M.F., 1991, Cold acclimation and cold regulated gene expression in ABA mutants of Arabidopsis thaliana, Plant Mol. Biol. 17: 1233–1240.
Gilmour, S.J., Zarka, D.G., Stockinger, E.J., Salazar, M.P., Houghton, J.M.. and Thomashow, M.F., 1998, Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an aerly step in cold-induced COR gene expression, Plant J. 16: 433–442.
Gosti, F., Beaudoin, N., Serizet, C., Webb, A.A.R., Vartanian, N. and Giraudat, J., 1999, ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling, Plant Cell 11:1897–1909.
Guy, C.L., 1990, Cold acclimation and freezing stress tolerance: role of protein metabolism. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41, 187–223.
Hasegawa, P.M., Bressan, R.A., Zhu, J-K. and Bohnert, H.J., 2000, Plant cellular and molecular responses to high salinity, Annu. Rev. Plant Physiol. Plant Mol. Biol. 51: 463–499.
Heino, P., Sandman, G., Lång, V., Nordin, K. and Palva, E.T., 1990, Abscisic acid deficiency prevents development of freezing tolerance in Arabidopsis thaliana (L.) Heynh, Theor. Appl. Genet. 79: 801–806.
Jaglo-Ottosen, K.R., Gilmour, S.J., Zarka, D.G., Schabenberger, O. and Thomashow, M., 1998, Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance, Science 280: 104–106.
Ketchum, K.A. and Slayman, C.W., 1996, Isolation of an ion channel gene from Arabidopsis thaliana using the H5 signature sequence from voltage-dependent K+ channels, FEBS Lett. 378: 19–26.
Kudla, J., Xu, Q., Harter, K., Gruissem, W. and Luan, S., 1999, Genes for calcineurin B-like proteins in Arabidopsis are differentially regulated by stress signals, Proc. Natl. Acad. Sci. USA 96: 4718–4723.
Kumori, T., and Yamamoto, M., 1994, Cloning of cDNAs from Arabidopsis thaliana that encode putative protein phosphatase 2C and human Dr-1-like protein by transformation of a fission yeast mutant, Nuc. Acid Res. 22: 5296–5301.
Lacombe, B., Pilot, G., Michard, E., Gaymard, F., Sentenac, H. and Thibaud, J-B., 2000, A shaker-like K+channel with weak rectifications is expressed in both source and sink phloem tissues of Arabidopsis, Plant Cell 12: 837–851.
Leube, M.P., Grill, E. and Amrheim, N., 1998, ABI1 of Arabidopsis is a protein serine/threonine phosphatse highly regulated by the proton and magnesium ion concentration, FEBS Lett. 424: 100–104.
Leung, J., Bouvier-Durand, M., Morris, P-C, Guerrier, D., Chefdor, F. and Giraudat, J., 1994, Arabidopsis ABA response gene ABI1: features of a calcium-modulated protein phosphatase, Science 264: 1448–1452.
Leung, J. and Giraudat, J., 1998, Abscisic acid signal transduction, Annu Rev. Plant Physiol. Plant Mol Biol. 49:199–222.
Leung, J., Merlot, S. and Giraudat, J., 1997, The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction, Plant Cell 9:759–771.
Liu, Q., Kasuga, M., Sakuma, Y., Abe, H., Miura, S., Yamaguchi-Shinozaki, K. and Shinozaki, K., 1998, Two transcription foctors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low temperature-responsive gene expression, respectively, in Arabidopsis, Plant Cell 10: 1391–1406.
Luan, S., 1998, Protein phosphatases and signaling cascades in higher plants, Trends Plant Sci. 3: 271–275.
Lång, V., Heino, P. and Palva, E.T., 1989, Low temperature acclimation and treatment with exogenous abscisic acid induce common polypeptides in Arabidopsis thaliana (L.) Heynh, Theor. Appl. Genet. 77: 729–734.
Lång, V., Mäntylä, E., Welin, B., Sundberg, B. and Palva, E.T., 1994, Alterations in water status, endogenous abscisic acid content, and expression of rab18 gene during the development of freezing tolerance in Arabidopsis thaliana, Plant Physiol. 104: 1341–1349.
Lång, V. and Palva, E.T., 1992, The expression of a rab-related gene, rab18, is induced by abscisic acid duringthe cold acclimation process of Arabidopsis thaliana (L.) Heynh, Plant Mol. Biol. 20: 951–962.
Maathuis, F.J.M., Ichida, A.M., Sanders, D. and Schroeder, J.I., 1997, Roles of higher plant K+ channels, Plant Physiol. 114: 1141–1149.
Marten, I., Hoth, S., Deeken, R., Ache, P., Ketchum, K.A., Hoshi, T. and Hedrich, R., 1999, AKT3, a phloem-localized K+ channel, is blocked by protons, Proc. Natl. Acad. Sci. USA 96: 7581–7586.
Merlot, S., Gosti, F., Guerrier, D., Vavasseur, A. and Giraudat, J., 2001, The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway, Plant J. 25: 315–324.
Meyer, K., Leube, M.P. and Grill, E., 1994, A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana, Science 264: 1452–1455.
Monroy, A.F., Sangwan, V. and Dhindsa, R.S., 1998, Low temperature signal transduction during cold acclimation: protein phosphatase 2A as an early target for cold-inactivation, Plant J. 13: 653–660.
Mäntylä, E., Lång, V. and Palva, E.T., 1995, Role of abscisic acid in drought-induced freezing tolerance, cold acclimation, and accumulation of LTI78 and RAB18 proteins in Arabidopsis thaliana, Plant Physiol. 107:141–148.
Nordin, K., Heino, P. and Palva, E.T., 1991, Separate signal pathways regulate the expression of a low-temperature-induced gene in Arabidopsis thaliana (L.) Heynh, Plant Mol. Biol. 16: 1061–1071.
Nordin, K., Vahala, T. and Palva, E.T., 1993, Differential expression of two related, low-temperature-induced genes in Arabidopsis thaliana (L.) Heynh, Plant Mol Biol. 21: 641–653.
Nylander, M., Heino, P., Helenius, E., Palva, E.T., Ronne, H. and Welin, B.V., 2001, The low-temperature- and salt-induced RCI2A gene of Arabidopsis complements the sodium sensitivity caused by a deletion of the homologous yeast gene SNA1, Plant Mol. Biol. 45: 341–352.
Sanders, D., Brownlee, C. and Harper, J.F., 1999, Communicating with calcium, Plant Cell 11: 691–706.
Sangwan, V., Foulds, I., Singh, J. and Dhindsa, R.S., 2001, Cold-activation of Brassica napus BN115 promoter is mediated by structural changes in membranes and cytoskeleton, and requires Ca2+ influx, Plant J. 27: 1–12.
Sheen, J., 1996, Ca2+-dependent protein kinases and stress signal transduction in plants, Science 274: 1900–1902.
Sheen, J., 1998, Mutational analysis of protein phosphatase 2C involved in abscisic acid signal transduction in higher plants, Proc. Natl. Acad. Sci. USA 95: 975–980.
Smith, R.D. and Walker, J.C., 1996, Plant Protein phosphatases, Annu. Rev. Plant Physiol. Plant Mol. Biol. 47:101–125.
Stockinger, E.J., Gilmour, S.J. and Thomashow, M., 1997, Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit, Proc. Natl. Acad. Sci. USA 94: 1035–1040.
Straub, P.F., Shen, Q. and Ho, T-H.D., 1994, Structure and promoter analysis of an ABA- and stress-regulated barley gene, HVA1, Plant Mol. Biol. 26: 617–630.
Tähtiharju, S. and Palva, T., 2001, Antisense inhibition of protein phosphatse 2C accelerates cold acclimation in Arabidopsis thaliana, Plant J. 26: 461–470.
Uno, Y., Furihata, T., Abe, H., Yoshida, R., Shinozaki, K. and Yamaguchi-Shinozaki, K., 2000, Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathways under drought and high-salinity conditions, Proc. Natl. Acad. Sci. USA 97: 11632–11637.
Vranová, E., Tähtiharju, S., Sriprang, R., Willekens, H., Heino, P., Palva, E.T., Inzé, D., and Van Camp, W., 2001, The AKT3 potassium channel protein interacts with the AtPP2CA protein phosphatase 2C, J. Exp. Bot. 52:181–182.
Wu, Y., Kuzma, J., Maréchal, E., Graeff, R., Lee, H.C., Foster, R. and Chua, N.-H., 1997, Abscisic acid signaling through cyclic ADP-ribose in plants, Science 278: 2126–2130.
Xu, Q., Fu, H-H., Gupta, R. and Luan, S., 1998, Molecular characterization of a tyrosine-specific protein phosphatase encoded by a stess-responsive gene in Arabidopsis, Plant Cell 10: 849–857.
Zimmermann, S., Ehrhardt, T., Plesch, G. and Müller-Röber, B., 1999, Ion channels in plant signaling, Cell. Mol. Life Sci. 55: 183–203.
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Tähtiharju, S., Heino, P., Palva, E.T. (2002). ATPP2CA Negatively Regulates ABA Responses during Cold Acclimation and Interacts with the Potassium Channel AKT3. In: Li, P.H., Palva, E.T. (eds) Plant Cold Hardiness. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0711-6_5
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DOI: https://doi.org/10.1007/978-1-4615-0711-6_5
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