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Cryoprotectin, A Cabbage Protein Protecting Thylakoids from Freeze-Thaw Damage

Expression of candidate genes in E. Coli

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Plant Cold Hardiness

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Abstract

We purified and partially sequenced a cryoprotective protein (cryoprotectin) from the leaves of cold-acclimated savoy cabbage (Brassica oleracea). Cryoprotectin protects thylakolds isolated from the leaves of non-acclimated spinach (Spinacia oleracea) from freeze-thaw damage. Sequencing of cryoprotectin revealed the copurification of at least two isoforms. The sequence data showed a high degree of similarity to a number of genes belonging to the class of lipid transfer proteins (LTP). The wax9 gene family of Brassica oleracea was chosen to clarify the function of individual genes with respect to cryoprotective activity. The five wax9 genes so far known were cloned and expressed in Escherichia coli. The preliminary data show for the first time that WAX9A and WAX9C have cryoprotective activity in an in vitro test assay. WAX9B and WAX9D had only low levels of cryoprotective activity while we could not detect any cryoprotective activity for WAX9E.

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Authors and Affiliations

  1. Institut für Pflanzenphysiologie, FU-Berlin, Königin-Luise-Str. 12-16a, 14195, Berlin, Germany

    Silke M. Schilling, Jürgen M. Schmitt & Carsten A. Köhn

  2. Biochemistry Department, Faculty of Agriculture, Ain-shams University, Cairo, Egypt

    Hany A. M. Sror

  3. Max-Planck Institut für Molekulare Pflanzenphysiologie, 14424, Potsdam, Germany

    Dirk K. Hincha

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  1. Silke M. Schilling
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  2. Hany A. M. Sror
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  3. Dirk K. Hincha
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  4. Jürgen M. Schmitt
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  5. Carsten A. Köhn
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Editors and Affiliations

  1. University of Minnesota, St. Paul, Minnesota, USA

    Paul H. Li

  2. University of Helsinki, Helsinki, Finland

    E. Tapio Palva

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Schilling, S.M., Sror, H.A.M., Hincha, D.K., Schmitt, J.M., Köhn, C.A. (2002). Cryoprotectin, A Cabbage Protein Protecting Thylakoids from Freeze-Thaw Damage. In: Li, P.H., Palva, E.T. (eds) Plant Cold Hardiness. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0711-6_14

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  • DOI: https://doi.org/10.1007/978-1-4615-0711-6_14

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