Adoptive transfer of mucosal T cells or dendritic cells from animals fed with cholera toxin B subunit alloantigen conjugate induces allogeneic T cell tolerance

  • Bin-Ling Li
  • Jia-Bin Sun
  • Jan Holmgren
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 495)


Repeated oral administration of allogeneic antigens (allo-Ag) in animals has been shown to delay rejection of cardiac allografts (1). However, oral tolerance induction by feeding allo-Ag is known to be ineffective to prevent rejection of full allogeneic grafts (1). We have previously found that oral administration of various antigens conjugated to the mucosa-binding molecule cholera toxin B subunit (CTB) can most effectively induce antigen-specific peripheral T cell tolerance (2), and also suppress several autoimmune diseases in experimental models (3). More recently, we and others reported that oral administration of a single dose of allogeneic antigens (allo-Ag) conjugated to CTB significantly prolongs murine allograft survival and inhibits the mixed lymphocyte reactions (MLR), allo-Ag-specific delayed-type hypersensitivity (DTH), as well as cell-mediated cytotoxicity (4 5).


Dendritic Cell Myelin Basic Protein Cholera Toxin Adoptive Transfer Mesenteric Lymph Node 
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  1. 1.
    Sayegh, M.H., Zhang, Z.J., Hancock, W.W., Kwok, C.A., Carpenter, C.B., and Weiner, H.L., 1992, Down-regulation of the immune response to histocompatibility antigen and prevention of sensitization by skin allografts by orally administrated alloantigens.Transplantation53:163–166.PubMedCrossRefGoogle Scholar
  2. 2.
    Sun, J.-B., Holmgren, J., and Czerkinsky, C., 1994, Cholera toxin B subunit: An effective transmucosal carrier-delivery system for induction of peripheral immunological tolerance.Proc. Natl. Acad. Sci. USA.91:10795–10799.PubMedCrossRefGoogle Scholar
  3. 3.
    Sun, J.-B., Rask, C., Olsson, T., Holmgren, J., and Czerkinsky, C., 1996, Treatment of experimental allergic encephalomyelitis by feeding myelin basic protein conjugated to cholera toxin B subunit.Proc. Natl. Acad. Sci. USA.93:196–7201.CrossRefGoogle Scholar
  4. 4.
    Ma, D., Mellon, J., and Niederkorn, J. Y., 1997, Oral immunization as a strategy for enhancing corneal allograft survival.British. J. Ophthalmology.81:778–784.CrossRefGoogle Scholar
  5. 5.
    Sun, J.-B., Li, B.-L., Czerkinsky, C., and Holmgren, J., 2000, Enhanced immunological tolerance against allograft rejection by oral administration of allogeneic antigen linked to cholera toxin B subunit.Clin. Immunol.97:130–139.PubMedCrossRefGoogle Scholar
  6. 6.
    Sun, J.-B., Xiao, B.-G., Lindblad, M., Li, B.-L., Link, H., Czerkinsky, C., and Holmgren, J., 2000, Oral administration of cholera toxin B subunit conjugated to myelin basic protein protects against experimental autoimmune encephalomyelitis by inducing TGFß, secreting cells and suppressing chemokine expression.Int. Immunol.12:1449–1457.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Bin-Ling Li
    • 1
  • Jia-Bin Sun
    • 1
  • Jan Holmgren
    • 1
  1. 1.Department of Medical Microbiology and ImmunologyGöteborg UniversityGöteborgSweden

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