Adoptive transfer of mucosal T cells or dendritic cells from animals fed with cholera toxin B subunit alloantigen conjugate induces allogeneic T cell tolerance
Repeated oral administration of allogeneic antigens (allo-Ag) in animals has been shown to delay rejection of cardiac allografts (1). However, oral tolerance induction by feeding allo-Ag is known to be ineffective to prevent rejection of full allogeneic grafts (1). We have previously found that oral administration of various antigens conjugated to the mucosa-binding molecule cholera toxin B subunit (CTB) can most effectively induce antigen-specific peripheral T cell tolerance (2), and also suppress several autoimmune diseases in experimental models (3). More recently, we and others reported that oral administration of a single dose of allogeneic antigens (allo-Ag) conjugated to CTB significantly prolongs murine allograft survival and inhibits the mixed lymphocyte reactions (MLR), allo-Ag-specific delayed-type hypersensitivity (DTH), as well as cell-mediated cytotoxicity (4 5).
KeywordsDendritic Cell Myelin Basic Protein Cholera Toxin Adoptive Transfer Mesenteric Lymph Node
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