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Molecular characterization of gut T cell precursors in euthymic and athymic mice

  • Florence Lambolez
  • Benedita Rocha
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 495)

Abstract

The major compartment of effector T cells in the body lines the epithelium of the murine gut. Intraepithelial T lymphocytes (T-IEL) from this location have large intracytoplasmic storage granules containing perforin and granzyme and express y-INF mRNA.Ex vivothey secrete this lymphokine and kill target cells immediately1 3. The number of these T cells is comparable to that of all T cells present in both spleen and lymph-nodes4. T-IEL have different origins in normal mice5-9. About half derive from activated T cells migrating from the peripheral lymphoid organs to the gut and have a memory phenotype (TcR-aß+CD5+CD28+LFA-1+CD44+CD8aß+or CD4+thymus-dependent-IEL/TD-IEL). The other half differs from peripheral T cells by phenotype (CD8aa+CD5-LFA-Y B220+) and CD3 complex expression (CD3-4 chain is associated with a analogue, the FcERIy chain10). They comprise a large proportion of TcR-y8+cells, and the TcR-aß+cells of this lineage are not deleted by self antigens4°1112. CD8aa T-IELs are thought to develop locally: structures named cryptopatches, disseminated randomly at the base of some gut crypts containing TcR-Thy1+c-kit+IL-7R+CD25+cells13,14were assimilated to “local” thymus like structures harboring differentiating precursors14,15Although CD8aa T-IELs are generated in athymic mice, the presence of a thymus increases their number by five-to 10 fold and modifies their composition5716. In euthymic mice, TcR-aß and TcR-y6 T cells are equally represented. In athymic mice about 90-95% of TcR+T cells are y8+. These differences remain after the injection of peripheral T cells(what reconstitutes TD-IEL), and thus are not due to any role of the TD-IEL on the maturation or expansion of CD8aa T-IEL.

Keywords

Athymic Mouse Intraepithelial Lymphocyte Chain Rearrangement Ectopic Thymus Euthymic Mouse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Florence Lambolez
    • 1
  • Benedita Rocha
    • 1
  1. 1.INSERM U.345France

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