Regulation of transporters associated with antigen processing (TAPs) by nucleotide binding to, and hydrolysis by, Walker consensus sequences

  • Loredana Saveanu
  • Peter M. van Endert
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 495)


The TAP transporters (TAPI/TAP2) belong to the large family of ATP­binding-cassette (ABC) transporters which translocate a variety of solutes across cellular membranes in an ATP-dependent fashion. ABC transporters are regulated by nucleotide triphosphate binding to, and hydrolysis by, several conserved sequence elements termed Walker A and B motifs which are located in the two hydrophilic ABC domains found in all members of the family. Nucleotide binding and hydrolysis are thought to be associated with sequential conformational changes that together constitute a transport cycle which starts with a high affinity substrate binding site on one side of the membrane and ends with a low affinity site on the other side (1).


Peptide Binding Nucleotide Binding Transport Cycle Nucleotide Hydrolysis Equivalent Mutation 
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  1. 1.
    Schneider, E., and Hunke, S. (1998). ATP-binding-cassette (ABC) transport systems: functional and structural aspects of the ATP-hydrolyzing subunits/domainsFEMS Microbiol. Rev.22, 1–20.PubMedCrossRefGoogle Scholar
  2. 2.
    van Endert, P. M. (1999). Role of nucleotides and peptide substrate for stability and functional state of the human ABC family transporters associated with antigen processingJ. Biol. Chem.274, 14632–8.PubMedCrossRefGoogle Scholar
  3. 3.
    Knittler, M. R., Alberts, P., Deverson, E. V., and Howard, J. C. (1999). Nucleotide binding by TAP mediates association with peptide and release of assembled MHC class I moleculesCurr. Biol.9, 999–1008.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Loredana Saveanu
    • 1
  • Peter M. van Endert
    • 1
  1. 1.Institut National de la Santé et de la Recherche Médicale Unité 25161 rue de SèvresParis Cedex 15France

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