Effects of Several Wine Polyphenols on Lipid Peroxidation and Oxygen Activation in Rat Liver Microsomes
Lipid peroxidation (LP) and reactive oxygen species (ROS) are thought to play important roles in atherosclerosis, cancer, ageing and other diseases1. Naturally occuring polyphenols in wine, tea, vegetables, etc can protect against these diseases by scavenging lipid radicals (R*, ROO*, RO*), O2radicals (O2-*, *OH), thereby preventing degradation of NO*, an important factor against atherosclerosis, by chelating transition metal ions thereby inhibiting the formation of free radicals2, by regeneration of vitamin C and E and (vi) by influencing various enzymes. They modulate lipoprotein metabolism and inhibit carcinogenesis1. In the microsomes, formation of ROS and LP is provided by cytochromes P450 and b5and P450 reductase.
KeywordsGallic Acid Caffeic Acid Lower Density Lipoprotein P450 Reductase Gentisic Acid
Unable to display preview. Download preview PDF.
- 2.C. Rice-Evans, Implications of the mechanisms of action of tea polyphenols as antioxidants in vitro chemoprevention in humans. P.S.E.B.M. 220:262 (1999).Google Scholar
- 3.I. Gut, V. Nededelcheva, P. Soucek, P. Stopka, B. Tichayska, Cytochromes P450 in benzene metabolism and involvement of their metabolites and reactive oxygen species in toxicity. Envir. Health Persp.104:1211 (1996).Google Scholar
- 4.O.H. Lowry, N.J.Rosenbrough, A.J. Fass, J.L. Randall, Protein measurement with the Folin Phenol reagent. J. Biol. Chem.193:265 (1951).Google Scholar
- 6.J.A.Buege, S.D. Aust, Microsomal lipid peroxidation. In: Methods in enzymology Vol 52 S. Fleischer, L. Packer ed. Academic Press, New York (1978).Google Scholar
- 10.J.A. Vinson, J. Jang, J. Yang, Y.A. Dabbagh, X. Liang, M.M. Serry, J. Proch, S. Cai, Vitamins and especially flavonoids in common beverages are powerful in vitro antioxidants which enrich lower density lipoproteins and increase their oxidative resistance after ex vivo spiking in human plasma. J. Agric. Food Chem.47:2502 (1999).PubMedCrossRefGoogle Scholar