Effects of Several Wine Polyphenols on Lipid Peroxidation and Oxygen Activation in Rat Liver Microsomes

  • Šárka Matějková
  • Ivan Gut
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 500)


Lipid peroxidation (LP) and reactive oxygen species (ROS) are thought to play important roles in atherosclerosis, cancer, ageing and other diseases1. Naturally occuring polyphenols in wine, tea, vegetables, etc can protect against these diseases by scavenging lipid radicals (R*, ROO*, RO*), O2radicals (O2-*, *OH), thereby preventing degradation of NO*, an important factor against atherosclerosis, by chelating transition metal ions thereby inhibiting the formation of free radicals2, by regeneration of vitamin C and E and (vi) by influencing various enzymes. They modulate lipoprotein metabolism and inhibit carcinogenesis1. In the microsomes, formation of ROS and LP is provided by cytochromes P450 and b5and P450 reductase.


Gallic Acid Caffeic Acid Lower Density Lipoprotein P450 Reductase Gentisic Acid 
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  1. 1.
    G.J. Soleas, E.P. Diamandis, D.M. Goldberg, Wine as a biological fluid: History, production and a role in disease prevention. J. Clin. Lab. Anal. 11:287 (1997).PubMedCrossRefGoogle Scholar
  2. 2.
    C. Rice-Evans, Implications of the mechanisms of action of tea polyphenols as antioxidants in vitro chemoprevention in humans. P.S.E.B.M. 220:262 (1999).Google Scholar
  3. 3.
    I. Gut, V. Nededelcheva, P. Soucek, P. Stopka, B. Tichayska, Cytochromes P450 in benzene metabolism and involvement of their metabolites and reactive oxygen species in toxicity. Envir. Health Persp.104:1211 (1996).Google Scholar
  4. 4.
    O.H. Lowry, N.J.Rosenbrough, A.J. Fass, J.L. Randall, Protein measurement with the Folin Phenol reagent. J. Biol. Chem.193:265 (1951).Google Scholar
  5. 5.
    T. Omura, R. Sato, The carbon monooxide-binding pigment of liver microsomes: evidence for its haemoproteine nature. J. Biol. Chem. 239:2370 (1964).PubMedGoogle Scholar
  6. 6.
    J.A.Buege, S.D. Aust, Microsomal lipid peroxidation. In: Methods in enzymology Vol 52 S. Fleischer, L. Packer ed. Academic Press, New York (1978).Google Scholar
  7. 7.
    S. Khan, R. Krishnamurthy, K.P. Padnya, Generation of hydroxyl radicals during benzene toxicity. Biochem. Pharmacol. 39:1393 (1990).PubMedCrossRefGoogle Scholar
  8. 8.
    S.A.B.E. van Acker, D.-J.van den Berg, M.N.L.J. Tromp, D.H. Griffionen, W.P. van Bennekom, W.J.F. Vijgh, A. Bast, Structural aspects of antioxidant activity of flavonoids. Free Rad. Biol. Med. 20:331 (1996).PubMedCrossRefGoogle Scholar
  9. 9.
    S.A.B.E. van Acker, G.P. van Balen, D.-J. van den Berg, A. Bast, W.J.F. van der Vijgh, Influence of iron chelation on the antioxidant activity of flavonoids. Biochem Pharm.56:935 (1998).PubMedCrossRefGoogle Scholar
  10. 10.
    J.A. Vinson, J. Jang, J. Yang, Y.A. Dabbagh, X. Liang, M.M. Serry, J. Proch, S. Cai, Vitamins and especially flavonoids in common beverages are powerful in vitro antioxidants which enrich lower density lipoproteins and increase their oxidative resistance after ex vivo spiking in human plasma. J. Agric. Food Chem.47:2502 (1999).PubMedCrossRefGoogle Scholar
  11. 11.
    Y.H. Miura, I. Tomita, T. Watanabe, T. Hirayama, S. Fukui, Active oxygens generation by flavonoids.Biol. Pharm. Bull. 21:93 (1998).CrossRefGoogle Scholar
  12. 12.
    Z.S. Zhao, S.Khan, P.J. O’Brien, Catecholic iron complexes as cytoprotective superoxid scavengers against hypoxia: reoxygenation injury in isolated hepatocytes. Biochem. Pharmacol. 56:825 (1998).PubMedCrossRefGoogle Scholar
  13. 13.
    I.B. Afanas’ev, A.I. Dorozhko A.V. Brodskii, V.A. Kostyuk, A.I. Potapovitch, Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation. Biochem. Pharmacol. 38:1763 (1989).PubMedCrossRefGoogle Scholar
  14. 14.
    J.F. Moran, R.J. Klucas, R.J. Grayer, J. Abian, M. Becana, Complexes of iron with phenolic compounds from soybean modules and other legume tissues: prooxidant and antioxidant properties. Free Rad. Biol. Med. 22:861 (1997).PubMedCrossRefGoogle Scholar
  15. 15.
    L. Belquendouz, L. Frémont, A. Linard, Resveratrol inhibits metal ion-dependent and independent peroxidation of porcine low-density lipoproteins. Biochem. Pharmacol. 53:1347 (1997).CrossRefGoogle Scholar
  16. 16.
    A. Arora, M. Nair, G.M. Strasburg, Structure-activity relationship for antioxidant activities of a series of flavonoids in a liposomal system. Free Rad. Biol Med. 24:1355 (1998).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Šárka Matějková
    • 1
  • Ivan Gut
    • 1
  1. 1.National Institute of Public HealthPraha 10Czech Republic

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