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HPLC Detection of Patulin in Apple Juice with GC/MS Confirmation of Patulin Identity

  • John A. G. Roach
  • Allan R. Brause
  • Thomas A. Eisele
  • Heidi S. Rupp
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 504)

Abstract

The official patulin LC procedure was further examined (AOAC 995.10). Juice or juice concentrate was extracted with ethyl acetate and cleaned up with sodium carbonate. Patulin in the dried extract was determined by reversed-phase LC with UV detection (280 nm) in 1% THE aqueous solution after evaporation of the ethyl acetate. An end-capped C18 column was required to separate patulin from hydroxymethylfurfural. Patulin was detected in approximately half of the >1000 extracts examined. Only ca 10% of the extracts contained patulin at levels greater than 50 µg/L (50 ppb). Some presumptive findings were confirmed by capillary gas chromatography/mass spectrometry as the trimethyl silyl derivative using electron ionization or as underivatized patulin using negative ion chemical ionization. Trifluoropropylmethyl polysiloxane capillary columns provided superior gas chromatography of underivatized patulin compared to phenyl/methyl polysiloxane and methyl polysiloxane columns.

Keywords

Apple Juice Juice Extract Stable Isotope Dilution Assay External Standard Curve Cider Vinegar 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • John A. G. Roach
    • 1
  • Allan R. Brause
    • 2
  • Thomas A. Eisele
    • 3
  • Heidi S. Rupp
    • 4
  1. 1.U.S. Food and Drug Administration
  2. 2.Analytical Chemical Services of Columbia Inc.Columbia
  3. 3.Treetop Inc.Selah
  4. 4.U.S. Food and Drug AdministrationBothell

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