Recent Advances in Analytical Methodology for Cyclopiazonic Acid

  • Joe W. Dorner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 504)

Abstract

Cyclopiazonic acid (CPA) is a toxic indole tetramic acid that has been isolated from numerous speciesofAspergillusandPenicillium.It has been found as a natural contaminant of cheese, corn, peanuts and various feedstuffs. Historically, thin-layer chromatography has been the most widely used method for quantitative determination of CPA in fungal cultures and agricultural commodities. Several liquid chromatographic (LC) and spectrophotometric methods have also been used, but these require extensive, time-consuming cleanup procedures to achieve accurate quantitation. More recently, enzyme-linked immunosorbent assays (ELISA) have been developed for quantification of CPA, and an immunoaffinity column (IAC) has been developed for cleanup of sample extracts prior to quantification by ELISA or LC. In applying the IAC to the cleanup of peanut extracts, recovery of CPA from spiked samples ranged from 83.7% to 90.8%, and the method was successfully applied to the analysis of peanuts that were naturally contaminated with CPA.

Keywords

Toxicity High Performance Liquid Chromatographic Corn Mold Sodium Hydroxide 

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Joe W. Dorner
    • 1
  1. 1.National Peanut Research LaboratoryUSDA, ARSSE Dawson

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