Summary
The use of confocal microscopy and fluorescent dyes permitted us to show that the nucleus plays an important role in excitation-contraction and secretion coupling of several excitable and non excitable cells. In addition, our group suggested that, like the sarcolemmal membrane, the nuclear envelope membrane possesses receptor s and channels which may play an important role in modulating Ca2+ influx and cytosolic Ca2+ buffering capacity of the nucleus.
Our results showed that the nucleus plays a role as a cytosolic Ca2+ buffer during spontaneous contraction of heart cells as well as during stimulation of cell surface membrane receptors of vascular endothelial and smooth muscle cells.
Using isolated nuclei of cardiomyocytes, vascular endothelial and smooth muscle cells, our results showed that receptors and channels are present at the nuclear envelope membranes. Our results also showed that receptors, such as Ang II and ET-1 receptors, undergo internalization and nuclear translocation and that the nucleus could be implicated in protein synthesis of these receptors. These results demonstrate that like the sarcolemmal membrane, the nuclear envelope membranes possesses channels and G-protein-coupled receptors that could be a new target for drug action.
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Bkaily, G., Jacques, D., Juste, P.D. (2004). Receptors and Channels of Nuclear Envelope Membranes as a New Target for Drug Action. In: Dhalla, N.S., Rupp, H., Angel, A., Pierce, G.N. (eds) Pathophysiology of Cardiovascular Disease. Progress in Experimental Cardiology, vol 10. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0453-5_33
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DOI: https://doi.org/10.1007/978-1-4615-0453-5_33
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