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Hypoxic Reperfusion after Brief Ischemia Potentiates Ischemic Preconditioning in Isolated Rat Hearts

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Myocardial Ischemia and Preconditioning

Part of the book series: Progress in Experimental Cardiology ((PREC,volume 6))

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Summary

Ischemic preconditioning renders the myocardium resistant to a subsequent sustained ischemic insult. The aim of this study was to investigate whether the reoxygenation that occurs during the intervening reperfusion of ischemic preconditioning is required for the development of cardioprotection. Isolated perfused rat hearts were either non-preconditioned (CONT) or preconditioned by 5 min ischemia and 5 min normoxic (IPCN) or hypoxic (IPCH) reperfusion before being subjected to 30 min total global normothermic ischemia followed by 30 min normoxic reperfusion. IPCN did not significantly alter the pre-ischemic status of tissue metabolites. However, after IPCH, ATP and glycogen levels were depressed and lactate content increased. As expected, IPCN protected the myocardium in terms of post-ischemic functional recovery (LVDP: IPCN = 53 ± 3% vs CONT = 31 ± 4%; p < 0.01). IPCH also improved contractile recovery (LVDP: 71 ± 5%, p < 0.01 vs CONT) and its cardioprotective effect was higher than that of IPCN (p < 0.05). At the end of reperfusion, tissue metabolite levels were not significantly different between IPCN and CONT groups while in IPCH group ATP level was significantly higher and AMP level was lower (p < 0.05 vs CONT). Our results show that i) during the intervening reperfusion of ischemic preconditioning, reoxygenation is not mandatory to achieve cardioprotection, and ii) a transient hypoxic reperfusion further enhances the beneficial effects of preconditioning against post-ischemic contractile dysfunction. We suggest that this latter phenomenon might be, at least in part, the consequence of glycogen depletion induced by the transient hypoxic reperfusion.

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Authors and Affiliations

  1. Laboratoire Stress Cardiovasculaires et Pathologies Associées, Université Joseph Fourier, Bâtiment Jean Roget—Domaine de La Merci, 38700, La Tronche, France

    Stéphane Tanguy, Sandrine Morel, François Boucher & Joël de Leiris

  2. Laboratoire des Régulations Physiologiques, Université de Kénitra, Maroc

    Nada Benajiba

Authors
  1. Marie-Claire Toufektsian
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  2. Stéphane Tanguy
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  3. Sandrine Morel
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  4. Nada Benajiba
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  5. François Boucher
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  6. Joël de Leiris
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Corresponding author

Correspondence to Joël de Leiris .

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Editors and Affiliations

  1. Institute of Cardiovascular Sciences St. Boniface General Hospital Research Centre Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla PhD, MD (Hon), DSc (Hon) (Distinguished Professor and Director) & Anton Lukas PhD (Associate Professor) (Distinguished Professor and Director) &  (Associate Professor)

  2. Aoto Hospital, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan

    Nobuakira Takeda MD, PhD (Associate Professor) (Associate Professor)

  3. Dept. of Pharmaceutical Sciences & Drug Research, Punjabi University, Patiala, India

    Manjeet Singh PhD (Professor & Head) (Professor & Head)

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Toufektsian, MC., Tanguy, S., Morel, S., Benajiba, N., Boucher, F., de Leiris, J. (2003). Hypoxic Reperfusion after Brief Ischemia Potentiates Ischemic Preconditioning in Isolated Rat Hearts. In: Dhalla, N.S., Takeda, N., Singh, M., Lukas, A. (eds) Myocardial Ischemia and Preconditioning. Progress in Experimental Cardiology, vol 6. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0355-2_16

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  • DOI: https://doi.org/10.1007/978-1-4615-0355-2_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5036-1

  • Online ISBN: 978-1-4615-0355-2

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