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The Application of Genetic Mouse Models to Elucidate a Role for Fibroblast Growth Factor-2 in the Mammalian Cardiovascular System

  • Chapter
Signal Transduction and Cardiac Hypertrophy

Part of the book series: Progress in Experimental Cardiology ((PREC,volume 7))

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Summary

Fibroblast growth factor (FGF)-2 is a polypeptide growth factor which plays multiple roles in the mammalian cardiovascular system, having direct proliferative, migratory and differentiation effects on cardiac myocytes, fibroblasts, smooth muscle and endothelial cells. To date, a number of genetic approaches in mice have been used to further our understanding of these roles and effects. The three main approaches used so far have been overexpression, gene disruption (“knockout”), and the use of a reporter gene for the study of transcriptional regulation in vivo. The application of genetic models offers the advantage of endogenous production, depletion or regulation as opposed to exogenously added growth factor or regulation studied ex vivo. The variety of approaches available has resulted in the publication of two overexpression models, four separate “knockout” models and one reporter gene system in transgenic mice. These models have been summarized in this review and will be discussed collectively in the context of the many roles for FGF-2 in the cardiovascular system, including development, cardioprotection, angiogenesis, blood pressure regulation, and hypertrophy. The reporter gene system will be highlighted in terms of the study of gene regulation in vivo. Finally, various means for fine-tuning transgenic systems will be considered with respect to the ability to control endogenous FGF-2 production more precisely.

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Authors and Affiliations

  1. Department of Anatomy, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, R3E 3J7, Canada

    Elissavet Kardami

  2. Department of Immunology, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, R3E 3J7, Canada

    Peter W. Nickerson

  3. Department of Physiology, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, R3E 3J7, Canada

    Dr. Peter A. Cattini

Authors
  1. Karen A. Detillieux
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  2. Sarah K. Jimenez
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  3. David P. Sontag
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  4. Elissavet Kardami
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  5. Peter W. Nickerson
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  6. Dr. Peter A. Cattini
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Correspondence to Peter A. Cattini .

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Editors and Affiliations

  1. Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla PhD, MD (Hon), DSc (Hon) (Distinguished Professor and Director), Larry V. Hryshko PhD (Associate Professor), Elissavet Kardami PhD (Professor) & Pawan K. Singal PhD, DSc (Professor) (Distinguished Professor and Director),  (Associate Professor),  (Professor) &  (Professor)

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Detillieux, K.A., Jimenez, S.K., Sontag, D.P., Kardami, E., Nickerson, P.W., Cattini, P.A. (2003). The Application of Genetic Mouse Models to Elucidate a Role for Fibroblast Growth Factor-2 in the Mammalian Cardiovascular System. In: Dhalla, N.S., Hryshko, L.V., Kardami, E., Singal, P.K. (eds) Signal Transduction and Cardiac Hypertrophy. Progress in Experimental Cardiology, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0347-7_27

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  • DOI: https://doi.org/10.1007/978-1-4615-0347-7_27

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5032-3

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