Abstract
Xenopus oocyte maturation offers a unique system for studying the non-genomic action of the steroid hormone progesterone. First and foremost, progesterone induces oocyte maturation through a pathway that does not require transcription (Masui and Markert, 19711). This is extremely important as progesterone’s non-transcriptional action can be studied under physiological conditions, contrasting the need to inhibit transcription in somatic cells. Second, immature oocytes are naturally synchronized at the diplotene stage (similar to G2 arrest in mitosis); and progesterone is the natural hormone that triggers re-initiation of oocyte maturation (Masui, 1967). Thus, the cytoplasmic signaling pathway activated by progesterone is a normal, physiological pathway whose downstream events are well-understood (Nebreda and Ferby, 2000). Third, the large size of oocytes (~1.5 mm diameter) means that individual oocytes can be easily manipulated. These include microinjection, enucleation, and various biochemical analyses of single oocytes.
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Liu, X.J., Ruderman, J.V. (2003). The Classical Progesterone Receptor Mediates Xenopus Oocyte Maturation Through a Non-Genomic Mechanism. In: Watson, C.S. (eds) The Identities of Membrane Steroid Receptors. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0339-2_11
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DOI: https://doi.org/10.1007/978-1-4615-0339-2_11
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