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Raman Spectroscopy for Whole Organism and Tissue Profiling

  • Chapter
Metabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis

Abstract

The inelastic light scattering process that has become eponymously known as Raman scattering was first observed in 1928 by the Indian physicist Chandrasekhara Venkata Raman and reported in Nature (Raman and Krishnan, 1928). Raman scattering spectra, like infrared (IR) absorption spectra, originate from an exchange of energy between photons and vibrational or rotational motions in molecules (Nelson, 1985). Raman scattering is a light scattering phenomenon in which an incident photon beam of well-defined wavelength (a monochromatic laser) is scattered by molecules. While most of the radiation is scattered elastically (Rayleigh scattering), a small fraction of the photons are modified as an irradiated molecule undergoes a vibrational transition (Adar et al., 1997).

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Author information

Authors and Affiliations

  1. Institute of Biologial Scicences University of Wales, Abersytwyth, SY23 3DD, UK

    Sarah Clarke & Royston Goodacre

  2. Department of Chemistry, University of Manchester Institute of Science, PO Box 88, Sackville St., Manchester, M60 1QD, UK

    Royston Goodacre

Authors
  1. Sarah Clarke
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  2. Royston Goodacre
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Editor information

Editors and Affiliations

  1. Pharmacia Corporation, Chesterfield, MO, 63198, USA

    George G. Harrigan

  2. Department of Chemistry, UMIST, PO Box 88, Manchester, M60 1QD, UK

    Royston Goodacre

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Clarke, S., Goodacre, R. (2003). Raman Spectroscopy for Whole Organism and Tissue Profiling. In: Harrigan, G.G., Goodacre, R. (eds) Metabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0333-0_6

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  • DOI: https://doi.org/10.1007/978-1-4615-0333-0_6

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