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Can 1H NMR Derived Metabolic Profiles Contribute to Proteomic Analyses?

A Study on Duchenne Muscular Dystrophy

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Metabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis

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Abstract

In the post genomic era, moving from the mapping of the genome of an organism to understanding its functional genomics is a massive undertaking. Many genes are not under translational control, suggesting that an approach based solely on transcriptomics is inadequate. Furthermore, analysis of the mammalian proteome remains an immense technical challenge. 1H nuclear magnetic resonance (NMR) derived metabolic profiles, however can define the phenotype of a pathology or genetic modification (Oliver, 2001; Gavaghan et al., 2001; Raamsdonk et al., 2001; Nicholson et al., 2002) providing a simple and new pathway into functional genomics.

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  1. Division of Biomedical Sciences, Imperial College of Science, Technology and Medicine, London, SW7 2A, UK

    Julian L. Griffin

  2. Department of Biochemistry, Oxford University, Oxford, OX1 3QV, UK

    Elizabeth Sang

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  1. Julian L. Griffin
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  1. Pharmacia Corporation, Chesterfield, MO, 63198, USA

    George G. Harrigan

  2. Department of Chemistry, UMIST, PO Box 88, Manchester, M60 1QD, UK

    Royston Goodacre

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Griffin, J.L., Sang, E. (2003). Can 1H NMR Derived Metabolic Profiles Contribute to Proteomic Analyses?. In: Harrigan, G.G., Goodacre, R. (eds) Metabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0333-0_3

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