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In vitro effect of nicotine and cotinine on the susceptibility of LDL oxidation and hemoglobin glycosylation

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Vascular Biochemistry

Abstract

Nicotine, a major component of cigarette smoke, plays an important role in the development of cardiovascular disease and lung cancer in smokers. This study was designed to determine the in vitro effects of nicotine and its metabolite cotinine on the susceptibility of LDL to oxidation and hemoglobin glycosylation. Three different concentrations of each one (10, 15, 25 µg/ ml) were used. Our data show that nicotine and cotinine are inhibitors for Cu2+-induced LDL oxidation but also they increase the glycosylation degree of hemoglobin. Nicotine at final concentrations of (10, 15, 25 µg/m1) increases the rate of hemoglobin glycosylation 25, 32 and 47%, respectively, and cotinine at final concentrations increase the rate of glycosylation 8, 10 and 12%, respectively. Therefore promoting hemoglobin glycosylation is one of the alternations caused by smoking that increase risk of cardiovascular disease. (Mol Cell Biochem 246: 117-120, 2003)

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Authors and Affiliations

  1. Department of Pharmacognosy, Isfahan University of Medical Science, Isfahan, Iran

    S. Asgary

  2. Department of Biochemistry, Isfahan University of Medical Science, Isfahan, Iran

    G. H. Naderi & M. Gharypur

  3. Cardiology, Isfahan Cardiovascular Research Center, Isfahan University of Medical Science, Isfahan, Iran

    N. Sarrafzadegan

Authors
  1. S. Asgary
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  2. G. H. Naderi
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  3. N. Sarrafzadegan
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  4. M. Gharypur
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Editor information

Editors and Affiliations

  1. Research Center, St. Boniface General Hospital, 351 Tache Avenue, Winnipeg, Manitoba, R2H 2A6, Canada

    Peter Zahradka

  2. Institute of Cardiovascular Sciences, St. Boniface General Hospital, 351 Tache Avenue, Winnipeg, Manitoba, R2H 2A6, Canada

    Jeffrey Wigle

  3. Division of Stroke & Vascular Disease, St. Boniface General Hospital Research Center, Canada

    Grant N. Pierce

  4. Faculty of Medicine, University of Manitoba, R2H 2A6, Winnipeg, Manitoba, Canada

    Grant N. Pierce

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© 2003 Springer Science+Business Media Dordrecht

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Asgary, S., Naderi, G.H., Sarrafzadegan, N., Gharypur, M. (2003). In vitro effect of nicotine and cotinine on the susceptibility of LDL oxidation and hemoglobin glycosylation. In: Zahradka, P., Wigle, J., Pierce, G.N. (eds) Vascular Biochemistry. Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease, vol 41. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0298-2_17

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  • DOI: https://doi.org/10.1007/978-1-4615-0298-2_17

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5010-1

  • Online ISBN: 978-1-4615-0298-2

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