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PACAP Receptor Knockout and Transgenics: What Have We Learnt?

  • Chapter
Pituitary Adenylate Cyclase-Activating Polypeptide

Part of the book series: Endocrine Updates ((ENDO,volume 20))

Abstract

Since the discovery of PACAP, originally isolated from hypothalamus in 1989 (Miyata et al, 1989), numerous studies devoted to the expression of this peptide have clearly demonstrated that it is not restricted to hypothalamic level but it is also expressed in various other brain areas and peripheral tissues. Functional studies have also established that the role of PACAP is not restricted to activation of the hypothalamic-pituitary complex, but that PACAP serves additional functions in the brain and peripheral organs. Most of these studies, herein reported in the precedent chapters, have been conducted both in vitro by incubation of PACAP, VIP and related peptides on either tumoral cell lines, primary cell cultures, or isolated perfused organs; and in vivo by injection of PACAP, or by administration of antagonists or PACAP antibodies to block the peptide activity. These approaches had the advantage of being technically simple, reproducible and/or relatively fast but were limited to a cellular or tissular context and to application of exogenous drugs. Recently, an alternative more appropriate approach to studying the physiology of PACAP has been the generation of mouse strains deficient in PACAP type 1 receptor and overexpressing the VIP (vasoactive intestinal peptide)/PACAP type 2 receptor (VPAC2).

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References

  • Aino H, Hashimoto H, Ogawa N, Nishino A, Yamamoto K, Nogi H, Nagata S, and Baba A. Structure of the gene encoding the mouse pituitary adenylate cyclase-activating polypeptide receptor. Gene 1995;164:301–304.

    Article  PubMed  CAS  Google Scholar 

  • Andersson A, Sundler F, and Ekblad E. Expression and motor effects of secretin in small and large intestine of the rat. Peptides 2000;21:1687–694.

    Article  PubMed  CAS  Google Scholar 

  • Arimura A, and Shioda S. Pituitary adenylate cyclase activating polypeptide (PACAP) and its receptors: neuroendocrine and endocrine interaction. Front Neuroendocrinol 1995;16:53–88

    Article  PubMed  CAS  Google Scholar 

  • Baron A, Monnier D, Roatti A, and Baertschi AJ. Pituitary adenylate cyclase-activating polypeptide activates K(ATP) current in rat atrial myocytes. Am J Physiol Heart Circ Physiol 2001;280:H1058–H1065.

    PubMed  CAS  Google Scholar 

  • Bertrand G, Puech R, Maisonnasse Y, Bockaert J, and Loubatieres-Mariani MM. Comparative effects of PACAP and VIP on pancreatic endocrine secretions and vascular resistance in rat. Br J Pharmacol 1996;117:764–770.

    Article  PubMed  CAS  Google Scholar 

  • Braas KM, May V, Harakall SA, Hardwick JC, and Parsons RL. Pituitary adenylate cyclaseactivating polypeptide expression and modulation of neuronal excitability in guinea pig cardiac ganglia. J Neurosci 1998;18(23):9766–9779.

    PubMed  CAS  Google Scholar 

  • Capecchi MR. Altering the genome by homologous recombination. Science 1989;244:1288–1292.

    Article  PubMed  CAS  Google Scholar 

  • Culler MD, and Paschall CS. Pituitary adenylate cyclase-activating polypeptide (PACAP) potentiates the gonadotropin-releasing activity of luteinizing hormone-releasing hormone. Endocrinology 1991;129:2260–2262.

    Article  PubMed  CAS  Google Scholar 

  • Daniel PB, Kieffer TJ, Leech CA, and Habener JF. Novel alternatively spliced exon in the extracellular ligand-binding domain of the pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC IR) selectively increases ligand affinity and alters signal transduction coupling during spermatogenesis. J Biol Chem 2001;27616:12938–12944.

    Article  Google Scholar 

  • Dickinson T, and Fleetwood-Walker SM. VIP and PACAP: very important in pain? Trends Pharmacol Sci 1999a;20:324–329.

    Article  CAS  Google Scholar 

  • Dickinson T, Mitchell R, Robberecht P, and Fleetwood-Walker SM. The role of VIP/PACAP receptor subtypes in spinal somatosensory processing in rats with an experimental peripheral mononeuropathy. Neuropharmacology 1999b;38:167–180.

    Article  CAS  Google Scholar 

  • Ekblad E, Ekelund M, and Sundler F. Relaxant responses of VIP and PACAP in rat ileum: receptors and adaptive supersensitivity. Ann N Y Acad Sci 1998;865:393–396.

    Article  PubMed  CAS  Google Scholar 

  • Ekblad E. Pharmacological evidence for both neuronal and smooth muscular PAC1 receptors and a VIP-specific receptor in rat colon. Regul Pept 1999;85:87–92.

    Article  PubMed  CAS  Google Scholar 

  • Ekblad E, Jongsma H, Brabet P, Bockaert J, and Sundler F. Characterization of intestinal receptors for VIP and PACAP in rat and in PAC1 receptor knockout mouse. Ann N Y Acad Sci 2000;921:137–147.

    Article  PubMed  CAS  Google Scholar 

  • Feany MB, and Quinn WG. A neuropeptide gene defined by the Drosophila memory mutant amnesiac. Science 1995;268:869–73.

    Article  PubMed  CAS  Google Scholar 

  • Filipsson K, Tornoe K, Holst J, and Ahren B. Pituitary adenylate cyclase-activating polypeptide stimulates insulin and glucagon secretion in humans. J Clin Endocrinol Metab 1997;82:3093–3098.

    Article  PubMed  CAS  Google Scholar 

  • Filipsson K, Pacini G, Scheurink AJ, and Ahren B. PACAP stimulates insulin secretion but inhibits insulin sensitivity in mice. Am J Physiol. 1998a;274:E834–842.

    CAS  Google Scholar 

  • Filipsson K, Sundler F, Hannibal J, and Ahren B. PACAP and PACAP receptors in insulin producing tissues: localization and effects. Regul Pept 1998b;74:167–175.

    Article  CAS  Google Scholar 

  • Filipsson K, Sundler F, and Ahren B. PACAP is an islet neuropeptide which contributes to glucose-stimulated insulin secretion. Biochem Biophys Res Commun 1999;256:664–667.

    Article  PubMed  CAS  Google Scholar 

  • Gonzalez, B. J., Basille, M., Vaudry, D., Fournier, A., and Vaudry, H. Pituitary adenylate cyclase-activating polypeptide promotes cell survival and neurite outgrowth in rat cerebellar neuroblasts. Neuroscience 1997;78:419–430.

    Article  PubMed  CAS  Google Scholar 

  • Gozes I, Glowa J, Brenneman DE, McCune SK, Lee E, and Westphal H. Learning and sexual deficiencies in transgenic mice carrying a chimeric vasoactive intestinal peptide gene. J Mol Neurosci 1993;4:185–193.

    Article  PubMed  CAS  Google Scholar 

  • Gressens P, Hill JM, Gozes I, Fridkin M, and Brenneman DE. Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos. Nature 1993;362:155–158.

    Article  PubMed  CAS  Google Scholar 

  • Grinevich V, Fournier A, and Pelletier G. Effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on corticotropin-releasing hormone (CRH) gene expression in the rat hypothalamic paraventricular nucleus. Brain Res 1997;773:190–196.

    Article  PubMed  CAS  Google Scholar 

  • Gu H, Marth JD, Orban PC, Mossmann H, and Rajewsky K. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 1994;265:103–106.

    Article  PubMed  CAS  Google Scholar 

  • Hannibal J, Ding JM, Chen D, Fahrenkrug J, Larsen PJ, Gillette MU, and Mikkelsen JD. Pituitary adenylate cyclase-activating peptide (PACAP) in the retinohypothalamic tract: a potential daytime regulator of the biological clock. J Neurosci 1997;17:2637–2644.

    PubMed  CAS  Google Scholar 

  • Hannibal J, Moller M, Ottersen OP, and Fahrenkrug J. PACAP and glutamate are co-stored in the retinohypothalamic tract. J Comp Neurol 2000;418:147–155.

    Article  PubMed  CAS  Google Scholar 

  • Hannibal J, Jamen F, Nielsen HS, Journot L, Brabet P, and Fahrenkrug J. Dissociation between light-induced phase shift of the circadian rhythm and clock gene expression in mice lacking the pituitary adenylate cyclase activating polypeptide type 1 receptor. J Neurosci 2001;21:4883–4890.

    PubMed  CAS  Google Scholar 

  • Hashimoto H, Shintani N, Nishino A, Okabe M, Ikawa M, Matsuyama S, Itoh K, Yamamoto K, Tomimoto S, Fujita T, Hagihara N, Mori W, Koyama Y, Matsuda T, Nagata S, and Baba A. Mice with markedly reduced PACAP (PAC(1)) receptor expression by targeted deletion of the signal peptide. J Neurochem 2000;75:1810–1817.

    Article  PubMed  CAS  Google Scholar 

  • Hirose M, Furukawa Y, Lakhe M, and Chiba S. Regional differences in cardiac effects of pituitary adenylate cyclase-activating polypeptide-27 in the isolated dog heart. Eur J Pharmacol 1998; 49:269–276.

    Article  Google Scholar 

  • Hosoya M, Kimura C, Ogi K, Ohkubo S, Miyamoto Y, Kugoh H, Shimizu M, OndaHOshimura M, Arimura A, and et al. Structure of the human pituitary adenylate cyclase activating polypeptide (PACAP) gene. Biochim Biophys Acta 1992;1129:199–206.

    Article  PubMed  CAS  Google Scholar 

  • Jamen F, Persson K, Bertrand G, Rodriguez-Henche N, Puech R, Bockaert J, Ahren B, and Brabet P. PAC1 receptor-deficient mice display impaired insulinotropic response to glucose and reduced glucose tolerance. J Clin Invest 2000a;105:1307–15.

    Article  CAS  Google Scholar 

  • Jamen F, Rodriguez-Henche N, Pralong F, Jegou B, Gaillard R, Bockaert J, and Brabet P. PAC1 null females display decreased fertility. Ann N Y Acad Sci 2000b;921:400–404

    Article  CAS  Google Scholar 

  • Jamen F, Puech R, Bockaert J, Brabet P, and Bertrand G. Etudes des voies de signalisation impliquees dans l’effet insulinotrope du PACAP dans les Ilots de rat et de souris deficientes ou non pour le recepteur PAC1. Diabetes and Metabolism 2001;27(Supplement to I).

    Google Scholar 

  • Jongsma H, Pettersson LM, Zhang Y, Reimer MK, Kanje M, Waldenstrom A, Sundler F, and Danielsen N. Markedly reduced chronic nociceptive response in mice lacking the PAC1 receptor. Neuroreport 2001;12:2215–2219.

    Article  PubMed  CAS  Google Scholar 

  • Kato I, Suzuki Y, Akabane A, Yonekura H, Tanaka O, Kondo H, Takasawa S, Yoshimoto T, and Okamoto H. Transgenic mice overexpressing human vasoactive intestinal peptide (VIP) gene in pancreatic beta cells. Evidence for improved glucose tolerance and enhanced insulin secretion by VIP and PHM-27 in vivo. J Biol Chem 1994;269:21223–21228.

    PubMed  CAS  Google Scholar 

  • Kondo T, Tominaga T, Ichikawa M, and Iijima T. Differential alteration of hippocampal synaptic strength induced by pituitary adenylate cyclase activating polypeptide-38 (PACAP-38). Neurosci Lett 1997;221:189–192.

    Article  PubMed  CAS  Google Scholar 

  • Kunzelmann P, Creff A, Bauer K, and Mollard P. PACAP and VIP induce changes in cytosolic calcium in putative folliculostellate cells of the mouse pituitary. Ann N Y Acad Sci 2000;921: 410–414.

    Article  PubMed  CAS  Google Scholar 

  • Lamouche S, and Yamaguchi N. Role of PAC(1) receptor in adrenal catecholamine secretion induced by PACAP and VIP in vivo. Am J Physiol Regul Integr Comp Physiol 2001;280:R510–R518.

    PubMed  CAS  Google Scholar 

  • Lu N, and DiCicco-Bloom E. Pituitary adenylate cyclase-activating polypeptide is an autocrine inhibitor of mitosis in cultured cortical precursor cells. Proc Natl Acad Sci USA 1997;94:3357–3362.

    Article  PubMed  CAS  Google Scholar 

  • Lutz EM, Sheward WJ, West KM, Morrow JA, Fink G, and Harmar AJ. The VIP2 receptor: molecular characterisation of a cDNA encoding a novel receptor for vasoactive intestinal peptide. FEBS Lett 1993;334:3–8.

    Article  PubMed  CAS  Google Scholar 

  • Martinez C, Delgado M, Pozo D, Leceta J, Calvo JR, Ganea D, and Gomariz RP. VIP and PACAP enhance IL-6 release and mRNA levels in resting peritoneal macrophages: in vitro and in vivo studies. J Neuroimmunol 1998;85:155–167.

    Article  PubMed  CAS  Google Scholar 

  • Masuo Y, Ohtaki T, Masuda Y, Tsuda M, and Fujino M. Binding sites for pituitary adenylate cyclase activating polypeptide (PACAP): comparison with vasoactive intestinal polypeptide (VIP) binding site localization in rat brain sections. Brain Res 1992;575:113–123.

    Article  PubMed  CAS  Google Scholar 

  • Masuo Y, Matsumoto Y, Tokito F, Tsuda M, and Fujino M. Effects of vasoactive intestinal polypeptide (VIP) and pituitary adenylate cyclase activating polypeptide (PACAP) on the spontaneous release of acetylcholine from the rat hippocampus by brain microdialysis. Brain Res 1993;611:207–215.

    Article  PubMed  CAS  Google Scholar 

  • Matsudaa K, Kashimoto K, Higuchi T, Yoshida T, Uchiyama M, Shioda S, Arimura A, and Okamura T. Presence of pituitary adenylate cyclase-activating polypeptide (PACAP) and its relaxant activity in the rectum of a teleost, the stargazer, Uranoscopus japonicus. Peptides 2000;21:821–827.

    Article  PubMed  CAS  Google Scholar 

  • Miyata A, Arimura A, Dahl RR, Minamino N, Uehara A, Jiang L, Culler MD, and Coy DH. Isolation of a novel 38 residue-hypothalamic polypeptide which stimulates adenylate cyclase in pituitary cells. Biochem Biophys Res Commun 1989;164:567–574.

    Article  PubMed  CAS  Google Scholar 

  • Nakata M, Shioda S, Oka Y, Maruyama I, and Yada T. Insulinotropin PACAP potentiates insulin-stimulated glucose uptake in 3T3 Ll cells. Peptides 1999;20:943–948.

    Article  PubMed  CAS  Google Scholar 

  • Nicot A, and DiCicco-Bloom E. Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression. Proc Natl Acad Sci USA 2001;98:4758–4763.

    Article  PubMed  CAS  Google Scholar 

  • Onaga T, Harada Y, and Okamoto K. Pituitary adenylate cyclase-activating polypeptide (PACAP) induces duodenal phasic contractions via the vagal cholinergic nerves in sheep. Regul Pept 1998;77:69–76.

    Article  PubMed  CAS  Google Scholar 

  • Otto C, Zuschratter W, Gass P, and Schutz G. Presynaptic localization of the PACAP-typelreceptor in hippocampal and cerebellar mossy fibres. Brain Res Mol Brain Res 1999;66:163–174.

    Article  PubMed  CAS  Google Scholar 

  • Otto C, Kovalchuk Y, Wolfer DP, Gass P, Martin M, Zuschratter W, Grone HJ, Kellendonk C, Tronche F, Maldonado R, Lipp HP, Konnerth A, and Schutz G. Impairment of mossy fiber long-term potentiation and associative learning in pituitary adenylate cyclase activating polypeptide type I receptor-deficient mice. J Neurosci 2001a;21:5520–5527.

    CAS  Google Scholar 

  • Otto C, Martin M, Paul Wolfer D, Lipp H, Maldonado R, and Schutz G. Altered emotional behavior in PACAP-type-I-receptor-deficient mice. Brain Res Mol Brain Res 2001b;92:78–84.

    Article  CAS  Google Scholar 

  • Pantaloni C, Brabet P, Bilanges B, Dumuis A, Houssami S, Spengler D, Bockaert J, and Journot L. Alternative splicing in the N-terminal extracellular domain of the pituitary adenylate cyclase-activating polypeptide (PACAP) receptor modulates receptor selectivity and relative potencies of PACAP-27 and PACAP-38 in phospholipase C activation. J Biol Chem 1996;271:22146–22151.

    Article  PubMed  CAS  Google Scholar 

  • Parker A, Meyer J, Lewitzky S, Rennich JS, Chan G, Thomas JD, Orho-Melander M, Lehtovirta M, Forsblom C, Hyrkko A, Carlsson M, Lindgren C, and Groop LC. A gene conferring susceptibility to type 2 diabetes in conjunction with obesity is located on chromosome 18p11. Diabetes 2001;50:675–680.

    Article  PubMed  CAS  Google Scholar 

  • Pellegri G, Magistretti PJ, and Martin JL. VIP and PACAP potentiate the action of glutamate on BDNF expression in mouse cortical neurones. Eur J Neurosci 1998;10:272–280.

    Article  PubMed  CAS  Google Scholar 

  • Pluja L, Fernandez E, and Jimenez M. Electrical and mechanical effects of vasoactive intestinal peptide and pituitary adenylate cyclase-activating peptide in the rat colon involve different mechanisms. Eur J Pharmacol 2000;389:217–224.

    Article  PubMed  CAS  Google Scholar 

  • Przywara DA, Kulkarni JS, Wakade TD, Leontiev DV, and Wakade AR. Pituitary adenylyl cyclase-activating polypeptide and nerve growth factor use the proteasome to rescue nerve growth factor-deprived sympathetic neurons cultured from chick embryos. J Neurochem 1998;71:1889–1897.

    Article  PubMed  CAS  Google Scholar 

  • Rawlings SR, Canny BJ, and Leong DA. Pituitary adenylate cyclase-activating polypeptide regulates cytosolic Ca2+ in rat gonadotropes and somatotropes through different intracellular mechanisms. Endocrinology 1993;132:1447–1452.

    Article  PubMed  CAS  Google Scholar 

  • Rawlings SR, and Hezareh M. Pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP/vasoactive intestinal polypeptide receptors: actions on the anterior pituitary gland. Endocr Rev 1996;17:4–29.

    PubMed  CAS  Google Scholar 

  • Sauvage M, Brabet P, Holsboer F, Bockaert J, and Steckler T. Mild deficits in mice lacking pituitary adenylate cyclase-activating polypeptide receptor type 1 (PAC1) performing on memory tasks. Brain Res Mol Brain Res 2000;84:79–89.

    Article  PubMed  CAS  Google Scholar 

  • Scaldaferri L, Arora K, Lee SH, Catt KJ, and Moretti C. Expression of PACAP and its type-I receptor isoforms in the rat ovary. Mol Cell Endocrinol 1996;117:227–232.

    Article  PubMed  CAS  Google Scholar 

  • Seebeck J, Schmidt WE, Kilbinger H, Neumann J, Zimmermann N, and Herzig S. PACAP induces bradycardia in guinea-pig heart by stimulation of atrial cholinergic neurones. Naunyn Schmiedebergs Arch Pharmacol 1996;354:424–430.

    Article  PubMed  CAS  Google Scholar 

  • Sekiguchi Y, Kasai K, Hasegawa K, Suzuki Y, and Shimoda S. Glycogenolytic activity of pituitary adenylate cyclase activating polypeptide (PACAP) in vivo and in vitro. Life Sci 1994;55:1219–1228.

    Article  PubMed  CAS  Google Scholar 

  • Shen S, Spratt C, Sheward WJ, Kallo I, West K, Morrison CF, Coen CW, Marston HM, and Harmar AJ. Overexpression of the human VPAC2 receptor in the suprachiasmatic nucleus alters the circadian phenotype of mice. Proc Natl Acad Sci USA 2000;97:11575–11580.

    Article  PubMed  CAS  Google Scholar 

  • Sheward WJ, Lutz EM, and Harmar AJ. The distribution of vasoactive intestinal peptide2 receptor messenger RNA in the rat brain and pituitary gland as assessed by in situ hybridization. Neuroscience 1995;67:409–418.

    Article  PubMed  CAS  Google Scholar 

  • Straub SG, and Sharp GW. A wortmannin-sensitive signal transduction pathway is involved in the stimulation of insulin release by vasoactive intestinal polypeptide and pituitary adenylate cyclase-activating polypeptide. J Biol Chem 1996;271:1660–1668.

    Article  PubMed  CAS  Google Scholar 

  • Sundler F, Ekblad E, Absood A, Hakanson R, Koves K, and Arimura A. Pituitary adenylate cyclase activating peptide: a novel vasoactive intestinal peptide-like neuropeptide in the gut. Neuroscience 1992;46:439–454.

    Article  PubMed  CAS  Google Scholar 

  • Takei N, Torres E, Yuhara A, Jongsma H, Otto C, Korhonen L, Abiru Y, Skoglosa Y, Schutz G, Hatanaka H, Sofroniew MV, and Lindholm D. Pituitary adenylate cyclase-activating polypeptide promotes the survival of basal forebrain cholinergic neurons in vitro and in vivo: comparison with effects of nerve growth factor. Eur J Neurosci 2000;12:2273–2280.

    Article  PubMed  CAS  Google Scholar 

  • Tolentino PJ, Dikkes P, Tsuruda L, Ebert K, Fink JS, Villa-Komaroff L, and Lamperti ED. Quantitative analysis of the expression of a VIP transgene. Brain Res Mol Brain Res 1995;33:47–60.

    Article  PubMed  CAS  Google Scholar 

  • van Gaalen M, Brabet P, Holsboer F, Bockaert J, and Steckler T. Increased anxiety-related behavior in mice lacking PACAP type I receptor. Society of Neuroscience 2000;Abstract(26):2042.

    Google Scholar 

  • van Gaalen MM, and Steckler T. Behavioural analysis of four mouse strains in an anxiety test battery. Behav Brain Res 2000;115:95–106.

    Article  PubMed  Google Scholar 

  • Vaudry D, Gonzalez BJ, Basille M, Yon L, Fournier A, and Vaudry H. Pituitary adenylate cyclase-activating polypeptide and its receptors: from structure to functions. Pharmacol Rev 2000;52:269–324.

    PubMed  CAS  Google Scholar 

  • Villalba M, Bockaert J, and Journot L. Pituitary adenylate cyclase-activating polypeptide (PACAP-38) protects cerebellar granule neurons from apoptosis by activating the mitogen-activated protein kinase (MAP kinase) pathway. J Neurosci 1997;17:83–90.

    PubMed  CAS  Google Scholar 

  • Wajima Z, Hua XY, and Yaksh TL. Inhibition of spinal protein kinase C blocks substance P-mediated hyperalgesia. Brain Res 2000;877:314–21.

    Article  PubMed  CAS  Google Scholar 

  • Waschek JA, Bravo DT, Sena M, Casillas R, Rodriguez W, Nguyen T, and Colbum S. Targeting of embryonic and postnatal autonomic and enteric neurons with a vasoactive intestinal peptide transgene. J Neurochem 1999;73:1739–48.

    Article  PubMed  CAS  Google Scholar 

  • Wei Y, and Mojsov S. Tissue specific expression of different human receptor types for pituitary adenylate cyclase activating polypeptide and vasoactive intestinal polypeptide: implications for their role in human physiology. J Neuroendocrinol 1996;8:811–817.

    Article  PubMed  CAS  Google Scholar 

  • Xu XJ, and Wiesenfeld-Hallin Z. Intrathecal pituitary adenylate cyclase activating polypeptide facilitates the spinal nociceptive flexor reflex in the rat. Neuroscience 1996;72:801–804.

    Article  PubMed  CAS  Google Scholar 

  • Yada T, Sakurada M, Ihida K, Nakata M, Murata F, Arimura A, and Kikuchi M. Pituitary adenylate cyclase activating polypeptide is an extraordinarily potent intra-pancreatic regulator of insulin secretion from islet beta-cells. J Biol Chem 1994;269:1290–1293.

    PubMed  CAS  Google Scholar 

  • Yada T, Sakurada M, Ishihara H, Nakata M, Shioda S, Yaekura K, Hamakawa N, Yanagida K, Kikuchi M, and Oka Y. Pituitary adenylate cyclase-activating polypeptide (PACAP) is an islet substance serving as an intra-islet amplifier of glucose-induced insulin secretion in rats. J Physiol 1997;505:319–328.

    Article  PubMed  CAS  Google Scholar 

  • Yokota C, Kawai K, Ohashi S, Watanabe Y, and Yamashita K. PACAP stimulates glucose output from the perfused rat liver. Peptides 1995;16:55–60.

    Article  PubMed  CAS  Google Scholar 

  • Zeng N, Athmann C, Kang T, Lyu RM, Walsh JH, Ohning GV, Sachs G, and Pisegna JR. PACAP type I receptor activation regulates ECL cells and gastric acid secretion. J Clin Invest 1999;104:1383–1391.

    Article  PubMed  CAS  Google Scholar 

  • Zhang Y, Malmberg AB, Sjolund B, and Yaksh TL. The effect of pituitary adenylate cyclase activating peptide (PACAP) on the nociceptive formalin test. Neurosci Lett 1996a;207:187–190.

    Article  CAS  Google Scholar 

  • Zhang YZ, Hannibal J, Zhao Q, Moller K, Danielsen N, Fahrenkrug J, and Sundler F. Pituitary adenylate cyclase activating peptide expression in the rat dorsal root ganglia: up-regulation after peripheral nerve injury. Neuroscience 1996b;74:1099–1110.

    CAS  Google Scholar 

  • Zhang Y, Danielsen N, Sundler F, and Mulder H. Pituitary adenylate cyclase-activating peptide is upregulated in sensory neurons by inflammation. Neuroreport 1998;9:2833–2836.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Unité Propre de Recherche 9023, Centre National de la Recherche Scientifique, Montpellier, 34094, France

    P. Brabet, F. Jamen, N. Rodriguez-Henche, G. Bertrand & J. Bockaert

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  1. P. Brabet
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  2. F. Jamen
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  3. N. Rodriguez-Henche
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  4. G. Bertrand
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  5. J. Bockaert
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  1. European Institute for Peptide Research, INSERM Unit 413, University of Rouen, Mont-Saint-Aignan, France

    Hubert Vaudry

  2. US Japan Biomedial Research Laboratories, Tulane University, Belle Chasse, Louisiana, USA

    Akira Arimura

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Brabet, P., Jamen, F., Rodriguez-Henche, N., Bertrand, G., Bockaert, J. (2003). PACAP Receptor Knockout and Transgenics: What Have We Learnt?. In: Vaudry, H., Arimura, A. (eds) Pituitary Adenylate Cyclase-Activating Polypeptide. Endocrine Updates, vol 20. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0243-2_15

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