Fiber Optic Immunosensors for Cardiovascular Disease Diagnosis

Quantification of Protein C, Factor V Leiden, and cardiac Troponin T in plasma
  • Hyun J. Kwon
  • Kyung A. Kang
  • Stephen C. Peiper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 510)

Abstract

Cardio-vascular diseases are principal causes of the mortality in the United States. These are closely associated with thrombophilia, and the most prevalent cause of the hereditary thrombophilia is the deficiency or dysfunction of hemostasis related plasma factors. Currently, there is an on-going effort to develop near real-time quantifying methods for the three important molecules related to cardio-vascular diseases in the Department of Chemical Engineering at the University of Louisville. They are: Protein C (PC), Factor V Leiden (FVL), and cardiac Troponin T (cTnT). PC is a key anticoagulant and anti-thrombotic in plasma at the normal concentration of 4 µg/ml. Activated Protein C (APC) achieves its anticoagulant role by inactivating Factor V (FV) and Factor VIII. The heterozygote (less than 60% of the normal level) has an 8-10 fold higher risk of the thrombotic complications by the age of 40 years.’ Currently, PC deficiency is diagnosed by enzyme linked immunosorbent assay (ELISA), which takes at least 6 hours to complete.

Keywords

Convection Albumin Immobilization Stratification Thrombin 

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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Hyun J. Kwon
    • 1
  • Kyung A. Kang
    • 1
  • Stephen C. Peiper
    • 2
  1. 1.Department of Chemical EngineeringUniversity of LouisvilleLouisville
  2. 2.James Graham Brown Cancer CenterUniversity of LouisvilleLouisville

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