Fiber Optic Immunosensors for Cardiovascular Disease Diagnosis
Cardio-vascular diseases are principal causes of the mortality in the United States. These are closely associated with thrombophilia, and the most prevalent cause of the hereditary thrombophilia is the deficiency or dysfunction of hemostasis related plasma factors. Currently, there is an on-going effort to develop near real-time quantifying methods for the three important molecules related to cardio-vascular diseases in the Department of Chemical Engineering at the University of Louisville. They are: Protein C (PC), Factor V Leiden (FVL), and cardiac Troponin T (cTnT). PC is a key anticoagulant and anti-thrombotic in plasma at the normal concentration of 4 µg/ml. Activated Protein C (APC) achieves its anticoagulant role by inactivating Factor V (FV) and Factor VIII. The heterozygote (less than 60% of the normal level) has an 8-10 fold higher risk of the thrombotic complications by the age of 40 years.’ Currently, PC deficiency is diagnosed by enzyme linked immunosorbent assay (ELISA), which takes at least 6 hours to complete.
KeywordsConvection Albumin Immobilization Stratification Thrombin
Unable to display preview. Download preview PDF.
- 1.Markis, M., Rosendaal, F. R., and Preston, F. E., Familial thrombophiloa:genetic risk factors and management, J. Internal Medicine 242 (Suppl. 740), 9–15 (1997).Google Scholar
- 4.Collinson, P., Stubbs, P., Moseley, D., Oakes, M., Greenwood, T., and Noble, M., Measurement of cardiac troponin T for risk stratification in unstable angina, Clin. Chem. 41 165 (1995).Google Scholar
- 5.Forest, J. C., Masse, J., and Lane, A., Evaluation of the analytical performance of the Boehringer Mannheim Elecsys 2010 immunoanalyzer, Clin. Biochem. 38 386–393 (1998).Google Scholar
- 6.Muller-Bardorff, M., Sylven, C., Rasmanis, G., Jorgensen, B., Collinson, P. 0., Waldenhofer, U., Hirsch!, M. M., Laggner, A. N., Gerhardt, W., Hafner, G., LabaereI. Lenberger, R., Zerback, R., and Katus H. A., Evaluation of a point-of-care system for quantitative determination of troponin T and myoglobin, Clin Chem. Lab. Med. 38 (6), 567–574 (2000).PubMedCrossRefGoogle Scholar
- 8.Kwon, H. J., Balcer, H. I., and Kang, K. A., Protein C biosensor sensitivity for biological samples and sensor reusability, Comparative Biochemistry and Physiology Part A, In press.Google Scholar