The P21-Activated Protein Kinases (Paks) Receive And Integrate Messages From A Variety of Signaling Pathways

  • Jian P. Lian
  • Lisa Crossley
  • Qian Zhan
  • RiYun Huang
  • Dwight Robinson
  • John A. Badwey
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 507)


Neutrophils stimulated with leukotriene B4, platelet activating factor or a variety of other chemoattractants that bind to G-protein coupled serpentine receptors exhibit rapid activation of two p21-activated kinases (Paks) with molecular masses of ca 63 and 69 kDa (y-and a- Pak)1.2.3 The 63 and 69 kDa Paks are ser/thr protein kinases that preferentially recognize the consensus sequence - (K/R)RX(S/T)- where X can be an acidic, basic or neutral amino acid4. These kinases can be conveniently assayed in neutrophils by their ability to undergo renaturation after separation by SDS/PAGE and undergoing autophosphorylation5 or catalyzing the phosphorylation of a peptide substrate fixed within a gel that corresponds to amino acid residues 297-331 of the 47 kDa subunit of the NADPH-oxidase complex (p47-phox) 1 .Histone H4 can also serve as a highly specific substrate for these enzymes in “in gel” assays5.


Myosin Light Chain Kinase Guanine Nucleotide Exchange Factor Sphingoid Base Signal Nuclear Translocation Candidate Target Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Jian P. Lian
    • 1
  • Lisa Crossley
    • 1
  • Qian Zhan
    • 1
  • RiYun Huang
    • 2
  • Dwight Robinson
    • 2
  • John A. Badwey
    • 1
  1. 1.Center for Experimental Therapeutics and Reperfusion Injury, Department of AnesthesiaBrigham and Women’s Hospital and the Harvard Medical SchoolBoston
  2. 2.Arthritis UnitMassachusetts General HospitalBoston

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