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Determination of Cyclooxygenase and Arachidonic Acid Metabolites in Invasive Human Prostate Cancer Cells

  • Kasem Nithipatikom
  • Christine L. Borscheid
  • Andre Kajdacsy-Balla
  • Sushma Kaul
  • Paul F. Lindholm
  • Kristen B. Pytynia
  • William B. Campbell
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 507)

Abstract

Cyclooxygenase (COX) metabolizes arachidonic acid (AA) to many biologically active eicosanoids such as prostaglandins (PGs), prostacyclins and thromboxane. Two forms of COX have been identified, a constitutive COX-1 and an inducible COX-2. COX-1 and COX-2 are expresséd from different gene (1) and utilize different pools of AA. COX-1 is involved in cell-cell signaling and maintaining tissue homeostasis. COX-2 is expressed in only certain cell types (2-3) and regulated by specific stimuli such as growth factors, tumor promoters and cytokines. It is hypothesized that COX-2 is responsible for the synthesis of prostanoids involved in pain, inflammation and mitogenesis (4-5). There is strong evidence that COX-2 and AA metabolites have important roles in the development, growth and invasiveness of many types of cancer. COX-2 expression increases PGE2production and metastatic potential of human breast cancer cell lines (6). PGF2awas found to enhance the invasiveness of mouse melanoma and human fibrosarcoma (7).

Keywords

Prostate Cancer Cell Human Breast Cancer Cell Line Human Prostate Cancer Cell Arachidonic Acid Metabolite SERS Intensity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Kasem Nithipatikom
    • 1
  • Christine L. Borscheid
    • 2
  • Andre Kajdacsy-Balla
    • 3
  • Sushma Kaul
    • 3
  • Paul F. Lindholm
    • 3
  • Kristen B. Pytynia
    • 4
  • William B. Campbell
    • 1
  1. 1.Department of Pharmacology and ToxicologyMedical College of WisconsinMilwaukeeWI
  2. 2.Department of ChemistryConcordia UniversitySt. PaulMN
  3. 3.Department of PathologyMedical College of WisconsinMilwaukeeWI
  4. 4.Department of Otolaryngology and Communication SciencesMedical College of WisconsinMilwaukeeWI

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