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cDNA Cloning and Mutagenesis Study of Liver-Type Prostaglandin F Synthase, and Identification of the Prostaglandin F Producing Cells in the Liver

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Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury, 5

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 507))

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Abstract

Prostaglandin F synthase (PGFS) is a multi-functional enzyme, and catalyzes the reduction of prostaglandin (PG)D2to 9a, 11f -PGF2and that of PGH2to PGF2aon the same molecule. PGFS has at least two isoformes, the lung-type enzyme’ (Km value of 120 µM for PGD2) and the liver-type one2(Km value of 10 µM for PGD2). The bovine lung-type PGFS is a:monomeric protein with a Mr of 36,666 consisting of 323 amino acids, and its amino acid sequence shows high homology compared with those of other aldo-keto reductase family (AKR family) members. However, the liver-type PGFS cDNA has not been isolated, and the primary structure has not been known. In the present study, we decided the primary structure of bovine liver-type PGFS and deduced the amino acids related the affinity for PGD2by the mutagenesis study.3Furthermore, our immunohistochemical study revealed PGF-producing cells in the liver.4

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References

  1. K. Watanabe, R. Yoshida, T. Shimizu, and O. Hayaishi, Enzymatic formation of prostaglandin FZafrom prostaglandin H2 and D2J Biol Chem.260:7035–7041 (1985).

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Author information

Authors and Affiliations

  1. Department of Anatomy and Cell Biology, School of Medicine, The University of Tokushima, 3-18-15 Kuramoto, Tokushima, 770-8503, Japan

    Toshiko Suzuki-Yamamoto, Hiromichi Yokoi, Yoshihiro Tsuruo & Kazunori Ishimura

  2. Fukui Medical School, 23-3 Shimoaizuki, Matsuoka, Yoshida, Fukui, 910-1103, Japan

    Yutaka Fujii

  3. Central Pharmaceutical Research Institute, Japan Tobacco, Inc., 1-1 Murasaki, Takatsuki, Osaka, 569-1125, Japan

    Masashi Miyano

  4. Department of Biochemistry, Cardiovascular Institute, Beijing, 100037, China

    Lan-Ying Chen

  5. Bioscience Research Laboratory, Mochida Pharmaceutical Co., 1-1-1 Kamiya, Kita, Tokyo, 115-0043, Japan

    Tomohiro Takahashi

  6. Division of Applied Life Sciences, Graduate School of Integrated Sciences and Arts, University of East Asia, Ichinomiyagakuen, Shimonoseki, 751-0807, Japan

    Kikuko Watanabe

Authors
  1. Toshiko Suzuki-Yamamoto
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  2. Yutaka Fujii
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  3. Masashi Miyano
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  4. Lan-Ying Chen
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  5. Tomohiro Takahashi
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  6. Hiromichi Yokoi
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  7. Yoshihiro Tsuruo
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  8. Kazunori Ishimura
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  9. Kikuko Watanabe
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Editor information

Editors and Affiliations

  1. Wayne State University, Detroit, Michigan, USA

    Kenneth V. Honn

  2. Vanderbilt University School of Medicine, Nashville, Tennessee, USA

    Lawrence J. Marnett

  3. Free University Berlin, Berlin, Germany

    Santosh Nigam

  4. University of California-San Diego, La Jolla, California, USA

    Edward Dennis

  5. Brigham and Women’s Hospital, Boston, Massachusetts, USA

    Charles Serhan

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© 2002 Springer Science+Business Media New York

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Suzuki-Yamamoto, T. et al. (2002). cDNA Cloning and Mutagenesis Study of Liver-Type Prostaglandin F Synthase, and Identification of the Prostaglandin F Producing Cells in the Liver. In: Honn, K.V., Marnett, L.J., Nigam, S., Dennis, E., Serhan, C. (eds) Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury, 5. Advances in Experimental Medicine and Biology, vol 507. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0193-0_40

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  • DOI: https://doi.org/10.1007/978-1-4615-0193-0_40

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4960-0

  • Online ISBN: 978-1-4615-0193-0

  • eBook Packages: Springer Book Archive

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