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cDNA Cloning and Mutagenesis Study of Liver-Type Prostaglandin F Synthase, and Identification of the Prostaglandin F Producing Cells in the Liver

  • Toshiko Suzuki-Yamamoto
  • Yutaka Fujii
  • Masashi Miyano
  • Lan-Ying Chen
  • Tomohiro Takahashi
  • Hiromichi Yokoi
  • Yoshihiro Tsuruo
  • Kazunori Ishimura
  • Kikuko Watanabe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 507)

Abstract

Prostaglandin F synthase (PGFS) is a multi-functional enzyme, and catalyzes the reduction of prostaglandin (PG)D2to 9a, 11f -PGF2and that of PGH2to PGF2aon the same molecule. PGFS has at least two isoformes, the lung-type enzyme’ (Km value of 120 µM for PGD2) and the liver-type one2(Km value of 10 µM for PGD2). The bovine lung-type PGFS is a:monomeric protein with a Mr of 36,666 consisting of 323 amino acids, and its amino acid sequence shows high homology compared with those of other aldo-keto reductase family (AKR family) members. However, the liver-type PGFS cDNA has not been isolated, and the primary structure has not been known. In the present study, we decided the primary structure of bovine liver-type PGFS and deduced the amino acids related the affinity for PGD2by the mutagenesis study.3Furthermore, our immunohistochemical study revealed PGF-producing cells in the liver.4

Keywords

Kupffer Cell Mutant Enzyme Mutagenesis Study Sinusoidal Endothelial Cell Apparent Homogeneity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    K. Watanabe, R. Yoshida, T. Shimizu, and O. Hayaishi, Enzymatic formation of prostaglandin FZafrom prostaglandin H2 and D2J Biol Chem.260:7035–7041 (1985).PubMedGoogle Scholar
  2. 2.
    L.Y. Chen, K. Watanabe, and O. Hayaishi, Purification and characterization of prostaglandin F synthase from bovine liverArch Biochem Biophys.296:17–26 (1992).PubMedCrossRefGoogle Scholar
  3. 3.
    T. Suzuki, Y. Fujii, M. Miyano, L.Y. Chen, T. Takahashi and K. Watanabe, cDNA cloning, expression, and mutagenesis study of liver-type prostaglandin F synthaseJ Biol Chem.274:241–248 (1999).PubMedCrossRefGoogle Scholar
  4. 4.
    T. Suzuki-Y., H. Yokoi, Y. Tsuruo, K. Watanabe, and K. Ishimura, Identification of prostaglandin F-producing cells in the liver, Histochem Cell Biol. 112:451–456(1999).Google Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Toshiko Suzuki-Yamamoto
    • 1
  • Yutaka Fujii
    • 2
  • Masashi Miyano
    • 3
  • Lan-Ying Chen
    • 4
  • Tomohiro Takahashi
    • 5
  • Hiromichi Yokoi
    • 1
  • Yoshihiro Tsuruo
    • 1
  • Kazunori Ishimura
    • 1
  • Kikuko Watanabe
    • 6
  1. 1.Department of Anatomy and Cell BiologySchool of Medicine, The University of TokushimaTokushimaJapan
  2. 2.Fukui Medical SchoolMatsuoka, Yoshida, FukuiJapan
  3. 3.Central Pharmaceutical Research InstituteJapan Tobacco, Inc.OsakaJapan
  4. 4.Department of BiochemistryCardiovascular InstituteBeijingChina
  5. 5.Bioscience Research LaboratoryMochida Pharmaceutical Co.TokyoJapan
  6. 6.Division of Applied Life SciencesGraduate School of Integrated Sciences and Arts, University of East AsiaShimonosekiJapan

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