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Cytosolic Phospholipase A2a Interacts With Microtubules

  • Yoshihito Nakatani
  • Sachiyo Sunaga
  • Makoto Murakami
  • Ichiro Kudo
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 507)

Abstract

Cytosolic phospholipase A2 (cPLA2) a plays crucial roles in the lipid mediator-biosynthetic pathway following cell activationl. Submicromolar concentrations of Ca2+ are required for cPLA2a to exert its catalytic activity, and this enzyme preferentially hydrolyses phospholipids bearing arachidonic acid2. cPLA2a exists in most mammalian cells, and its activation is regulated by several postreceptor signal transduction events, such as Ca2+ mobilization3, phosphorylation4,5, and gene induction6. Upon Ca2+mobilizing stimulation, cPLA2a translocates rapidly to the perinuclear area7-9. cPLA2a has several functionally distinct regions: an amino-terminal Ca2+-dependent lipid-binding domain (amino acids 18-138) called the C2 domainl0, a carboxy-terminal region (amino acids 179-749) containing the catalytic domain, a putative pleckstrin homology domain 11, and two critical serine residues (Ser505 and Ser727), which undergo activation-directed phosphorylation12. We recently demonstrated that the C2 domain of cPLA2 interacts with vimentin, a major component of intermeiate filaments, in a Ca2+dependent mannerl3. In this report, we have found that another cytoskeletal protein in the cytosol of fibroblasts binds to the C2 domain of cPLA2a.

Keywords

Intermediate Filament Microtubule Assembly Arachidonic Acid Release Cytosolic Phospholipase Perinuclear Area 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Yoshihito Nakatani
    • 1
  • Sachiyo Sunaga
    • 1
  • Makoto Murakami
    • 1
  • Ichiro Kudo
    • 1
  1. 1.Department of Health ChemistrySchool of Pharmaceutical Sciences, Showa UniversityTokyoJapan

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