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Interactions Between 5-Oxo-Ete and Chemokines in Stimulating Eosinophils

  • William S. Powell
  • Sylvie Gravel
  • Joshua Rokach
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 507)

Abstract

Cells containing 5-lipoxygenase convert arachidonic to both LTA4-derived leukotrienes and 5-HETE (Fig. 1). Although 5-HETE has biological effects on inflammatory cells that are not mediated by LT receptors, it is not very potentI. However, 5-HETE is enzymatically oxidized to a product, 5-oxo-ETE, that is 100-fold more potent than its precursor in activating neutrophils2. The enzyme responsible for the formation of 5-oxo-ETE, 5-hydroxyeicosanoid dehydrogenase, is highly specific for eicosanoids containing a 5S-hydroxyl group followed by a6-transdouble bond3. Thus 5S-HETE is its preferred substrate, whereas LTB4, 5R-HETE, and other HETEs undergo little or no metabolism3. The high degree of specificity exhibited by this enzyme was the first clue that its product, 5-oxo-ETE, may serve some biological function. 5Hydroxyeicosanoid dehydrogenase is present in a variety of leukocytes, including neutrophils3, eosinophils4, monocytes, and lymphocytes. However, formation of 5-oxoETE depends not only on the presence of this enzyme, but also requires adequate levels of the cofactor NADP+ 5. Thus resting neutrophils are not very active in converting 5HETE to 5-oxo-ETE, whereas neutrophils that have been stimulated to undergo the oxidativburst by addition of phorbol myristate acetate or opsonized zymosan produce substantial amounts of 5-oxo-ETE due to the activation of NADPH oxidase.

Keywords

Human Neutrophil Actin Polymerization Calcium Mobilization Phorbol Myristate Acetate Potent Stimulator 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • William S. Powell
    • 1
  • Sylvie Gravel
    • 1
  • Joshua Rokach
    • 2
  1. 1.Meakins-Christie Laboratories, Department of MedicineMcGill UniversityMontrealCanada
  2. 2.the Claude Pepper Institute and Department of ChemistryFlorida Institute of TechnologyMelbourneUSA

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