Leukotriene D4-Induced Calcium Signaling in Human Intestinal Epithelial Cells
LTD4induces a calcium signal that consists of a mobilization from internal stores regulated by PTX-insensitive G protein, Rho, and, an influx across the plasma membrane regulated by PTX-sensitive Gi protein in human intestinal epithelial cells. The LTD4induced mobilization of Ca2+is mediated by a PH domain dependent association between PLC-71 and Gßy subunits. This interaction requires Src kinase activity as well as the associtation of this kinase with PLC-71, suggesting a G(37 mediated recruitment of proteins to the plasma membrane and formation of a signaling complex which is essential for the downstream Ca2+ signal. We found a cAMP-dependent protein kinase activity upstream of tyrosine kinase(s) and downstream of Gi protein, that is essential for LTD4induced Ca2+ influx. This model of the LTD4-induced Ca2+signaling pathways in intestinal epithelial cells is outlined schematically.
KeywordsHuman Epithelial Cell Human Intestinal Epithelial Cell Human Epithelial Cell Line Growth Factor Signal Transduction Permeable cAMP Analog
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