Leukotriene D4-Induced Calcium Signaling in Human Intestinal Epithelial Cells

  • Charles Kumar Thodeti
  • Anita Sjölander
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 507)


LTD4induces a calcium signal that consists of a mobilization from internal stores regulated by PTX-insensitive G protein, Rho, and, an influx across the plasma membrane regulated by PTX-sensitive Gi protein in human intestinal epithelial cells. The LTD4induced mobilization of Ca2+is mediated by a PH domain dependent association between PLC-71 and Gßy subunits. This interaction requires Src kinase activity as well as the associtation of this kinase with PLC-71, suggesting a G(37 mediated recruitment of proteins to the plasma membrane and formation of a signaling complex which is essential for the downstream Ca2+ signal. We found a cAMP-dependent protein kinase activity upstream of tyrosine kinase(s) and downstream of Gi protein, that is essential for LTD4induced Ca2+ influx. This model of the LTD4-induced Ca2+signaling pathways in intestinal epithelial cells is outlined schematically.


Human Epithelial Cell Human Intestinal Epithelial Cell Human Epithelial Cell Line Growth Factor Signal Transduction Permeable cAMP Analog 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Charles Kumar Thodeti
    • 1
  • Anita Sjölander
    • 1
  1. 1.Department of Laboratory Medicine, Division of Experimental PathologyLund University, University Hospital MalmöSweden

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