Intracellular Activation and Induction of 12-lipoxygenase in Mouse Peritoneal Macrophages

  • Hiroo Kawajiri
  • Isamu Makino
  • Tanihiro Yoshimoto
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 507)


Upon macrophage activation, arachidonic acid is released from membrane lipids, and metabolized by cyclooxygenase (COX) and lipoxygenases.1,2 COX is the rate-limiting enzyme for the synthesis of various prostaglandins and thromboxanes, and there are two isoforms, COX-1 and COX-2.3 COX-1 is constantly expressed in resting macrophages, while COX-2 is rapidly and transiently induced by various stimuli such as cytokines and lipopolysaccharide (LPS).4 Prostaglandin E2 regulates macrophage functions including syntheses of nitric oxide5 and cytokines.6 On the other hand, 12-lipoxygenase introduces a molecular oxygen into arachidonic acid to give rise its 12-hydroperoxy derivative.7 It has been shown that this enzyme is responsible for the generation of oxidized low-density lipoprotein which is an initial event of atherosclerosis.8.9 Therefore, it is of great importance to examine intracellular activation of the 12-lipoxygenase in macrophages. For this purpose, we utilized a dichlorofluorescein dye to sensitively visualize the enzyme activity, and found that LPS induced the 12-lipoxygenase in mouse peritoneal macrophages.


Arachidonic Acid Intracellular Activation Mouse Peritoneal Macrophage Tumor Necrosis Factor Production Nordihydroguaiaretic Acid 
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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Hiroo Kawajiri
    • 1
  • Isamu Makino
    • 1
  • Tanihiro Yoshimoto
    • 1
  1. 1.Department of PharmacologyKanazawa University School of MedicineKanazawaJapan

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