Advertisement

Substrate Down Regulation of 12-lipoxygenase in Human Platelets

  • Marina Dadaian
  • Pär Westlund
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 507)

Abstract

Lipoxygenases are non heme iron containing enzymes which catalyze dioxygenation of unsaturated fatty acids with the production of the corresponding hydroperoxides. The main mammalian lipoxygenases are classified with respect to the positional specificity of the oxygenation of arachidonic acidI. 12-Lipoxygenase (12-LO) from human platelets was the first mammalian lipoxygenase described2however, the biological significance of this pathway in platelets is not completely understood. Furthermore, little is known about regulation of 12-lipoxygenase activity in human platelets. It has been shown that 12-LO is activated by its product, 12-hydroperoxyeicosatetraenoic acid (12-HPETE)3.

Keywords

Arachidonic Acid Human Platelet Heme Iron Calcium Ionophore A23187 Positional Specificity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    S. Yamamoto, Mammalian lipoxygenases:molecular structures and functionsBiochint. Biophys. Acta.1128: 117 (1992).CrossRefGoogle Scholar
  2. 2.
    M. Hamberg, and B. Samuelsson, Prostaglandin endoperoxides. Novel transformations of arachidonic acid in human plateletsProc. Natl. Acad. Sci. USA.71:3400 (1974).PubMedCentralPubMedCrossRefGoogle Scholar
  3. 3.
    M.1. Siegel, R.T. McConnell, S.L. Abrahams, N.A. Porter, and P. Cuatrecasas, Regulation of arachidonate metabolism via lipoxygenase and cyclo-oxygenase by 12-HPETE, the product of human platelet lipoxygenaseBiochem. Biophys. Res. Commun.89:1273 (1979).PubMedCrossRefGoogle Scholar
  4. 4.
    R.L. Maas, and A.R. Brash, Evidence for a lipoxygenase mechanism in the biosynthesis of epoxide and dihydroxy leukotrienes from 15(S)-hydroperoxyicosatetraenoic acid by human platelets and porcine leukocytesProc. Natl. Acad. Sci. USA.80:2884 (1983).PubMedCentralPubMedCrossRefGoogle Scholar
  5. 5.
    J.Y. Vanderhoek, R.W. Bryant, and J. M. Bailey, 15-Hydroxy-5,8,11,13-eicosatetraenoic acid.A potent and selective inhibitor of platelet lipoxygenaseJ. Biol. Chem.255:5996 (1980).PubMedGoogle Scholar
  6. 6.
    M. Dadaian, and P. Westlund, Albumin modifies the metabolism of hydroxyeicosatetraenoic acids via 12 lipoxygenase in human plateletsJ. Lipid Res.40:940 (1999).PubMedGoogle Scholar
  7. 7.
    S.T. Prigge, J.C.Boyington, M. Faig, K.S. Doctor, B.J. Gaffney, and L.M. Amzel, Structure and mechanism of lipoxygenasesBiochimie.79:629 (1997).Google Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Marina Dadaian
    • 1
  • Pär Westlund
    • 1
  1. 1.Department of Woman and Child Health Division of Reproductive EndocrinologyKarolinska HospitalStockholmSweden

Personalised recommendations