6-Nitrotryptophan: A Specific Reaction Product Of Tryptophan Residue In Human Cu, Zn-Sod Treated With Peroxynitrite

  • Fumiyuki Yamakura
  • Takashi Matsumoto
  • Hikari Taka
  • Tsutomu Fujimura
  • Kimie Murayama
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 527)


Human Cu,Zn-superoxide dismutase (SOD) was treated with peroxynitrite. The modified enzyme was digested by trypsin. Decrease of one peptide and appearance of three new peptides (A, B, C) with increase molecular weights of 16, 16, and 45 compared to that of the decreased peptide, respectively, were observed by LC/MS analysis. These increased molecular weights correspond to the addition of one oxygen and nitro group, respectively. Among these new peptides, the peptide C showed thesame visible absorption spectrum as that of 6-nitrotryptophan. We conclude 6-nitrotryptophan is one of the reaction products between tryptophan residue in Cu, Zn-SOD and peroxynitrite.


Nitric Oxide Nitro Group Tryptic Peptide Tryptophan Residue Ammonium Bicarbonate 
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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Fumiyuki Yamakura
    • 1
  • Takashi Matsumoto
    • 2
  • Hikari Taka
    • 3
  • Tsutomu Fujimura
    • 3
  • Kimie Murayama
    • 3
  1. 1.Department of ChemistryJuntendo University School of MedicineJapan
  2. 2.Department of Food Science and NutritionShowa Women’s UniversitySetagaya, TokyoJapan
  3. 3.Centaral Laboratory of Medical SciencesJuntendo University School of MedicineTokyoJapan

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