Potassium Hexacyanoferrate(Iii)-Mediated Fluorometric Determination of Melatonin

  • Hideaki Iizuka
  • Takehiko Yajima
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 527)


This paper describes the development of a novel, simple and sensitive high-performance liquid chromatographic method for the determination of melatonin using precolumn fluorometric derivatization. A strong fluorescence was induced when alkaline solution of melatonin was heated in the presence of potassium hexacyanoferrate (III). This fluorescent reaction was used for the precolumn derivatization of melatonin. Solid phase extraction was used for concentrating melatonin below a level of detection limit.


Solid Phase Extraction Column Native Fluorescence Precolumn Derivatization Potassium Hexacyanoferrate Acetonitrile Concentration 
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© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Hideaki Iizuka
  • Takehiko Yajima

There are no affiliations available

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