Potassium Hexacyanoferrate(Iii)-Mediated Fluorometric Determination of Melatonin
This paper describes the development of a novel, simple and sensitive high-performance liquid chromatographic method for the determination of melatonin using precolumn fluorometric derivatization. A strong fluorescence was induced when alkaline solution of melatonin was heated in the presence of potassium hexacyanoferrate (III). This fluorescent reaction was used for the precolumn derivatization of melatonin. Solid phase extraction was used for concentrating melatonin below a level of detection limit.
KeywordsHydrogen Peroxide HPLC Hydroxide Acetonitrile Tryptophan
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