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Plasma Leptin, Insulin And Free Tryptophan Contribute To Cytokine-Induced Anorexia

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Developments in Tryptophan and Serotonin Metabolism

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 527))

Abstract

Cytokines contribute to anorexia of diseases. Tumor Necrosis Factor (TNF) and/or interleukin-1 (IL-1) stimulate leptin release, but not insulin. Both affect hypothalamus to decrease food intake (FI). Hypothalamic serotonin (5HT) decreases FI. Its synthesis depends on brain availability of precursor, tryptophan (TRP), which depends on plasma free TRP. Purpose is to test involvement of plasma leptin, insulin, TRP, and thus hypothalamic 5HT in cytokine-induced anorexia in rats. In male rats, IL-1 a (10 mg/kg/d; n=9), TNFa (30 mg/kg/d; n=9), Il-la+TNFa (10:30 mg/kg/d; n=9), TRP (100 mg/kg/d, n=8) and saline (n=8; Control) were injected sc for 2 days. FI, BW, plasma free and total TRP, leptin and insulin, and body fat were measured. Data analyzed via ANOVA. IL-la and IL-la+TNFa vs others decreased FI and BW. TNFa and TRP did not change FI and BW. Plasma total TRP was higher in TRP vs IL-la, TNFa, and IL-la+TNFa. Plasma free TRP was higher in IL-la and IL- l a+TNFa vs Control. IL-la and IL- l a+TNFa decreased leptin and body fat. Insulin in Control was lower than others. Data suggest: i) IL-la increases plasma free TRP, but not total TRP, thus increases hypothalamic 5HT synthesis, resulting in anorexia; ii) leptin does not mediate anorexia, but; iii) insulin may contribute to anorexia induced by cytokines.

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Authors and Affiliations

  1. Surgical Metabolism and Nutrition Laboratory, Department of Surgery, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY, 13210

    Tomoi Sato

  2. Department of Clinical Medicine, University of Rome “La Sapienza”, Rome, Italy

    Alessandro Laviano

  3. Department of Clinical Medicine, University of Rome “La Sapienza”, viale dell’Università 37, 00185, Rome, Italy

    MichaelM Meguid

  4. Department of Surgery, Niigata University School of Medicine, Niigata, 951-8510, Japan

    FilippoRossi Fanelli

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  1. Tomoi Sato
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  2. Alessandro Laviano
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  3. MichaelM Meguid
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  4. FilippoRossi Fanelli
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Editor information

Editors and Affiliations

  1. University of Padova, Padova, Italy

    Graziella Allegri , Carlo V. L. Costa  & Eugenio Ragazzi ,  & 

  2. University of Hamburg, Hamburg, Germany

    Hans Steinhart

  3. Istituto G. Gaslini, Genova Quarto, Italy

    Luigi Varesio

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Sato, T., Laviano, A., Meguid, M., Fanelli, F. (2003). Plasma Leptin, Insulin And Free Tryptophan Contribute To Cytokine-Induced Anorexia. In: Allegri, G., Costa, C.V.L., Ragazzi, E., Steinhart, H., Varesio, L. (eds) Developments in Tryptophan and Serotonin Metabolism. Advances in Experimental Medicine and Biology, vol 527. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0135-0_27

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  • DOI: https://doi.org/10.1007/978-1-4615-0135-0_27

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4939-6

  • Online ISBN: 978-1-4615-0135-0

  • eBook Packages: Springer Book Archive

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